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文献和实验Imaging B-Cell Receptor Signaling by Single-Molecule Techniques
. Recognition of antigens by the B-cell antigen receptor (BCR) leads to microclustering of the BCR and the initiation of intracellular signaling that prompts the B cells to stop and form immunological synapses with the antigen-presenting cells
spreading, we developed a method engaging integrin signaling by use of an immobilized anti-integrin monoclonal antibody (mab) directed against the fibronectin (FN) receptor integrin α5β1. ECV 304 cells were plated onto FN or immobilized mab JBS5 (anti
All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
O2 in methanol for 10 minutes. Wash slides 3 times with 1X PBS for 2 minutes, each time and then proceed to primary antibody incubation. 3. Epitope Retrieval Protocol A sample epitope retrieval protocol developed for some Zymed® Antibodies
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