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文献和实验1. Pick single colony and inoculate 5 ml of LB broth containing 200 g/l ampicillin or 1mg/5ml. Optional: Use a 15ml conical tube with a loosened cap and a piece of tape to hold it in place. Shake at 250 RPM 37℃ overnight. 2. Centrifuge 1.5mL cells
. Optional: Use a 15ml conical tube with a loosened cap and a piece of tape to hold it in place. Shake at 250 RPM 37℃ overnight. 2. Centrifuge 1.5mL cells in 1.5 mL Eppendorf tube at top speed for 1 minute. Aspirate supernatant. 3. Resuspend
Transplantation of Pancreatic Islets Into the Kidney Capsule of Diabetic Mice
and tape the PE50 tubing over the side of the 5 mL pipet to prevent curling of the PE50 tubing while centrifuging. Centrifuge the PE50/15mL conicals to 1000 rpm and turn off centrifuge. (Never prepare more than 10 islet preparations at one time
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