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- 2025年10月27日
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Nuvia IMAC Resin, based on Nuvia Beads, contains nitrilotriacetic acid (NTA) as the chelating ligand for di- or trivalent metal ions. The chemical structure of NTA permits highly selective binding of recombinant histidine-tagged proteins when charged with Ni2+ or other transition metals, such as Zn2+ or Cu2+.
Due to superior mass transfer characteristics, Nuvia IMAC Resin offers high capacity at high flow rates. The resin can be used under either nondenaturing or denaturing conditions.
Features and Benefits of Nuvia IMAC Resin
Best-in-class binding capacity at fast flow rates
Rapid mass transfer kinetics
Efficient resolution of biomolecules from crude feedstreams
Stable at pH 2–14, providing excellent NaOH stability
Applications of Nuvia IMAC Resin
For purifying biomolecules in a variety of workflows:
Excellent single-step purity via affinity chromatography
Quick, efficient separation of biomolecules
Contaminant removal
Robust base matrix
This IMAC resin is compatible with 8 M urea, 6 M guanidine HCl, 1 M NaOH, alcohol up to 20% (v/v), and with common detergents, salts, reducing agents, and all commonly used aqueous buffers.
Packaging Options
Bulk Nuvia IMAC Resin
25 ml (780-0800)
100 ml (780-0801)
500 ml (780-0802)
Prepacked Nuvia™ IMAC Columns
Use these 5 ml cartridges with the NGC™ Chromatography System or any low- to medium-pressure chromatography system.
More Information
Download the Nuvia IMAC Resin Instruction Manual for detailed column packing recommendations and protocols for optimal protein isolation and column regeneration, cleaning, sanitizing, and storage.
Related Products
View all Affinity Chromatography Resins.
Empty chromatography columns
Spin columns
Low-pressure EconoColumn™ Columns
Medium-pressure columns
Desalting columns for secondary purification
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文献和实验conditions are the same for all washes unless otherwise stated. Wash the Ni-NTA resin three times each with 30 µL of 100 mM EDTA (pH 8.0) to remove the bound Ni2+ metal. Wash the column three times each with 30 µL of H2O. Wash the column three times
Adhere 介质为复合多模式填料，其配基含有阴离子交换，氢键和疏水性质，对于抗体的聚集体有非常独特而高效的去除能力，此外通过有效的实验设计（DoE），还可以起到有效去除脱落的Protein A 配基，宿主蛋白（HCP），核酸，内毒素以及潜在的病毒，且通过流穿模式时，Capto Adhere 载量可达 100-200mg/ml，将 Mabselect SuRe LX 和 Capto Adhere 相结合，仅用两步层析纯化，就可以得到符合药用级质量要求的高纯度抗体产品，大大缩短了工艺时间，提高生产效率
Characterization of Phosphopeptides Using a Combination of Immobilized Metal Ion
unless otherwise stated. Wash the Ni-NTA resin three times each with 30 µL of 100 mM EDTA (pH 8.0) to remove the bound Ni2+ metal. Wash the column three times each with 30 µL of H2O. Wash
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