Lane 1: Mouse Cerebrum tissue lysates Lane 2: Rat Cerebrum tissue lysates Lane 3: Rat Cerebellum tissue lysates Lane 4: Human Hela cell lysates Lane 5: Human SH-SY5Y cell lysates Lane 6: Human Jurkat cell lysates Lane 7: Human Huvec cell lysates using beta tubulin antibody at 1/20000 dilution
Western blot analysis of Hela Lysate using Beta tubulin at antibody 1/2000~1/20000 dilution.
Western blot analysis of Cerebrum Lysate using Beta tubulin antibody at 1/1000~1/20000 dilution.
Western blot analysis of Hela,NIH/3T3,HL60 Cerebellum,Cerebrum using beta tubulin antibody at 1/1000 dilution.
IHC
IHC analysis of fixed, paraffin- embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with beta tubulin antibody.
IHC analysis of fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with beta tubulin Antibody.