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- 询价记录
- 文献和实验
- 技术资料
- 英文名：
123count eBeads
- 供应商：
研卉生物
- 规格：
10 * 2^10（100 tests ）
● 在流式平台上计数细胞
● 利用抗体标记细胞并利用123count eBeads计算该细胞群数量
● 可同时并用FVD细胞存活染料标记活细胞计数
123count eBeads™应用领域
● 全血细胞计数
● 流式细胞仪计数功能验证
● 精子浓度计数
● 可并用细胞存活染料7-AAD, PI或FVD计数细胞存活率
● 使用磁珠细胞分选试剂盒后计算细胞分选产率与纯度
货号：01-1234-42 , 100个反应
123count eBeads™使用方式
● 加入123count eBeadsTM→选定待计数的细胞 →公式计算细胞浓度。
● 9加入*已知浓度的123count eBeads 进未知浓度的细胞样本
● 流式细胞仪同时读取细胞与123count eBeads
● 公式计算细胞浓度如下：
Whole blood was stained with CD45 PE, CD3 FITC and CD4 APC, and then lysed with 1-step Fix/Lyse Solution (10X)(cat. no. 00-5333). 123count eBeads™ were added after lysis. Lymphocytes were gated on CD45+ cells,
and then analyzed for CD3+ and CD4+ cells (left). 123count eBeads were counted based on bright fluorescence in FITC and PerCP-eFluor® 710 (right).
全血CD4实际细胞计数示范
= (流式细胞仪显示细胞数量/流式细胞仪显示eBead数量) X eBead标示浓度
= (541个细胞/1009 eBeads) X 998 eBeads/ul
= 535个细胞/ul
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文献和实验Exercise 12.7 - Viability Cell Count Materials Suspension culture of cells Sterile transfer pipettes Stock 0.2% (w/v) Trypan blue Hemacytometer and microscope Procedure Gently
HOW TO USE THE COULTER COUNTER TO COUNT CELLS
any dilution, as long as you know how to multiply. 2) Be sure to mix the sample thoroughly before counting, since the cells will settle to the bottom. To count the sample, turn the glass valve directly above the cells to vertical (open). Wait
Viability Cell Count Materials Suspension culture of cells Sterile transfer pipettes Stock 0.2% (w/v) Trypan blue Hemacytometer and microscope Procedure Gently
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