Whole blood was stained with CD45 PE, CD3 FITC and CD4 APC, and then lysed with 1-step Fix/Lyse Solution (10X)(cat. no. 00-5333). 123count eBeads™ were added after lysis. Lymphocytes were gated on CD45+ cells,
and then analyzed for CD3+ and CD4+ cells (left). 123count eBeads were counted based on bright fluorescence in FITC and PerCP-eFluor® 710 (right).
全血CD4实际细胞计数示范 = (流式细胞仪显示细胞数量/流式细胞仪显示eBead数量) X eBead标示浓度 = (541个细胞/1009 eBeads) X 998 eBeads/ul = 535个细胞/ul