AIM2 Antibody

8055:

Western Blotting

AIM2 Antibody recognizes endogenous levels of total AIM2 protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro250 of human AIM2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from HL-60 cells, serum-starved and either untreated (-) or treated overnight with Human Interferon-γ (hIFN-γ) #8901 (100 ng/ml), using AIM2 Antibody.

AIM2 (absent in melanoma 2) is an interferon-inducible protein containing an amino-terminal pyrin domain and carboxy-terminal HIN-200 domain that functions in innate immunity and tumor progression (1). Expression of AIM2 can inhibit cell growth and tumor formation (2,3). Furthermore, the gene for AIM2 shows a high frequency of mutations associated with microsatellite unstable colorectal cancers (4). AIM2 has a critical role in the activation of caspase-1, the protease responsible for the processing of pro-inflammatory cytokines IL-1β and IL-18. Caspase-1 activation is regulated by multi-protein complexes referred to as “inflammasomes” (5,6). Distinct inflammasome complexes have been described containing NLRP1/NALP1, NLRP3/NALP3, IPAF, and AIM2. The HIN-200 domain of AIM2 is responsible for binding to cytoplasmic double stranded DNA, resulting in caspase-1 activation. (7-9). This inflammasome complex also involves binding of the pyrin domain of AIM2 to the CARD-domain protein ASC/TMS1, which then interacts directly with caspase-1. As a result, AIM2 has been demonstrated to be an important sensor for a number of different pathogens (10-12).

1. DeYoung, K.L. et al. (1997) Oncogene 15, 453-7.
2. Chen, I.F. et al. (2006) Mol Cancer Ther 5, 1-7.
3. Patsos, G. et al. (2010) Int J Cancer 126, 1838-49.
4. Woerner, S.M. et al. (2007) Genes Chromosomes Cancer 46, 1080-9.
5. Schroder, K. and Tschopp, J. (2010) Cell 140, 821-32.
6. Khare, S. et al. (2010) Crit Rev Immunol 30, 463-87.
7. Roberts, T.L. et al. (2009) Science 323, 1057-60.
8. Hornung, V. et al. (2009) Nature 458, 514-8.
9. Fernandes-Alnemri, T. et al. (2009) Nature 458, 509-13.
10. Jones, J.W. et al. (2010) Proc Natl Acad Sci USA 107, 9771-6.
11. Fernandes-Alnemri, T. et al. (2010) Nat Immunol 11, 385-93.
12. Kim, S. et al. (2010) Eur J Immunol 40, 1545-51.

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• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.