- 询价
- Cell Signaling Technology
- 5399
- USA
- 2025年10月22日
- W, IP, IF-IC
- Rabbit
- H,M,R
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- 详细信息
- 技术资料
- 抗体英文名：
Phospho-eIF4B (Ser406) Antibody
- 抗原：
synthetic peptide corresponding to residues surrounding Ser406 of human eIF4B protein
- 应用范围：
W, IP, IF-IC
- 宿主：
Rabbit
- 级别：
详见MSDS文件
- 供应商：
CST
- 适应物种：
H,M,R
- 保质期：
详见说明书
- 库存：
大量
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC | H M (R) | Endogenous | 80 | Rabbit |
| Protocols | |
|---|---|
| Specificity / Sensitivity | Phospho-eIF4B (Ser406) Antibody detects endogenous levels of eIF4B protein only when phosphorylated at Ser406. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser406 of human eIF4B protein. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, untreated or λ phosphatase treated, using Phospho-eIF4B (Ser406) Antibody (upper) or eIF4B Antibody #3592 (lower). IF-IC
Confocal immunofluorescent analysis of HeLa cells, untreated (upper) or λ phosphatase treated (lower), using Phospho-eIF4B (Ser406) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye). |
| Background | Eukaryotic initiation factor 4B (eIF4B) is thought to assist the eIF4F complex in translation initiation. In plants, eIF4B is known to interact with the poly-(A) binding protein, increasing its poly-(A) binding activity (1). Heat shock and serum starvation cause dephosphorylation of eIF4B at multiple sites with kinetics similar to those of the corresponding inhibition of translation, while phosphorylation of eIF4B following insulin treatment correlates well with an observed increase in translation (2-5). Multiple kinases, including p70 S6 kinase, can phosphorylate eIF4B in vitro , and at least one serum-inducible eIF4B phosphorylation site is sensitive to rapamycin and LY294002 (6). Recently, Ser406 was identified as a novel phosphorylation site regulated by mitogens (7), and the phosphorylation of this site is dependent on MEK and mTOR activity (7). This phosphorylation is shown to be essential for the translational activity of eIF4B (7).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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