- 询价
- Cell Signaling Technology
- 3470
- USA
- 2025年11月26日
- W, IHC-P, IF-IC
- Rabbit
- M,H
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- 详细信息
- 技术资料
- 抗体英文名：
Perilipin (D418) Antibody
- 抗原：
synthetic peptide corresponding to the sequence around Asp418 of human perilipin
- 应用范围：
W, IHC-P, IF-IC
- 宿主：
Rabbit
- 库存：
大量
- 保质期：
详见说明书
- 供应商：
CST
- 级别：
详见MSDS文件
- 适应物种：
M,H
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P IF-IC | M (H) | Endogenous | 62 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Perilipin (D418) Antibody detects endogenous levels of total perilipin protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence around Asp418 of human perilipin. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from NIH/3T3 and differentiated 3T3-L1 cells using Perilipin (D418) Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded 3T3-L1 cells, control (left) or differentiated (right), using Perilipin (D418) Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse brown fat using Perilipin (D418) Antibody in the presence of control peptide (left) or antigen-specific peptide (right). IF-IC
Confocal immunofluorescent analysis of differentiated 3T3-L1 cells using Perilipin (D418) Antibody (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | Triacylglycerol is stored in lipid droplets as a primary energy reserve. Perilipin is localized at the periphery of lipid droplets and serves as a protective coating against lipases (1-3). Evidence suggests that PKA regulates lipolysis by phosphorylating perilipin (1,2,4,5). Phosphorylation of perilipin results in the conformational change that exposes lipid droplets to endogenous lipases, such as hormone-sensitive lipases (2). Hence, perilipin plays a pivotal role in lipid storage (2,5). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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