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- 详细信息
- 文献和实验
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- 供应商:
赛信通(上海)生物试剂有限公司暨美国CST中国分公司
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1 Kit
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文献和实验步骤 1. Plate preparation 1) Dilute capture antibody (polyclonal) with PBS to a concentration of 1μg/ml. Immediately, add 100μl to each ELISA plate well. Seal the plate and incubate overnight at room temperature. (Monoclonal Antibody –
using primary and secondary antibodies Sandwich ELISA protocol using a pair of primary antibodies Flow cytometry / FACS protocols: Direct flow cytometry (FACS) protocol Indirect flow cytometry (FACS) protocol Immunoprecipitation (IP) protocols
are discussed in the following sections. Covering of epitopes Under certain circumstances, blocking reagents can interfere with the correct detection of analytes. This phenomenon is rarely observed in sandwich assay formats with a capture antibody
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