MCM3 Antibody

4012:

IHC / Paraffin , Immunofluorescence , Immunoprecipitation , Western Blotting

MCM3 Antibody detects endogenous levels of total MCM3 protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human MCM3. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from various cell types using MCM3 Antibody.

IP

Immunoprecipitation of MCM3 from HeLa cell lysates using MCM3 Antibody followed by western blot using the same antibody. Lane 1 is 5% input.

IHC-P (paraffin)

Immunohistochemcial analysis of paraffin-embedded human colon carcinoma using MCM3 Antibody.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using MCM3 Antibody.

IF-IC

Confocal immunofluorescent analysis of HeLa cells using MCM3 Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).

The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for the initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is also a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication (reviewed in 2). Phosphorylation of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis (6-8). MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Licensing of the chromatin permits the DNA to replicate only once per cell cycle, thereby helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). Studies have shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest (reviewed in 3,9).

1. Lei, M. and Tye, B.K. (2001) J Cell Sci 114, 1447-54.
2. Lygerou, Z. and Nurse, P. (2000) Science 290, 2271-3.
3. Forsburg, S.L. (2004) Microbiol Mol Biol Rev 68, 109-31.
4. Tye, B.K. and Sawyer, S. (2000) J Biol Chem 275, 34833-6.
5. Labib, K. et al. (2000) Science 288, 1643-7.
6. Charych, D.H. et al. (2008) J Cell Biochem 104, 1075-86.
7. Masai, H. et al. (2006) J Biol Chem 281, 39249-61.
8. Lin, D.I. et al. (2008) Proc Natl Acad Sci USA 105, 8079-84.
9. Bailis, J.M. et al. (2008) Mol Cell Biol 28, 1724-38.

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For Research Use Only. Not For Use In Diagnostic Procedures.