- 询价
- Cell Signaling Technology
- 3070
- USA
- 2025年07月15日
- W
- Rabbit
- R,H,M
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- 详细信息
- 技术资料
- 抗体英文名：
Phospho-IRS-1 (Tyr895) Antibody
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Tyr896 of human IRS-1
- 应用范围：
W
- 宿主：
Rabbit
- 供应商：
CST
- 保质期：
详见说明书
- 库存：
大量
- 级别：
详见MSDS文件
- 适应物种：
R,H,M
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | R (H) (M) | Transfected Only | 180 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-IRS-1 (Tyr895) Antibody detects transfected levels of IRS-1 only when phosphorylated at Tyr895. The antibody may cross-react with other activated receptor tyrosine kinases (RTKs) and docking proteins. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr896 of human IRS-1. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from CHO cells stably transfected with IRS-1, untreated or stimulated with insulin, using Phospho-IRS-1 (Tyr895) Antibody (upper) or IRS-1 Antibody #2382 (lower). |
| Background | Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10). Phosphorylation of Tyr895 in IRS-1 provides a binding site for Grb2, which mediates the downstream signaling leading to MAP kinase activation and mitogenesis (11).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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