- 询价
- Cell Signaling Technology
- 5166
- USA
- 2026年01月07日
- W, IF-IC
- H,M,R,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
EAPP (1E4) Mouse mAb
- 抗原：
recombinant amino-terminal fragment of human EAPP protein
- 应用范围：
W, IF-IC
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 库存：
大量
- 供应商：
CST
- 适应物种：
H,M,R,Mk
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IF-IC | H M R Mk | Endogenous | 45 | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | EAPP (1E4) Mouse mAb detects endogenous levels of total EAPP protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a recombinant amino-terminal fragment of human EAPP protein. Western Blotting
Western blot analysis of extracts from various cell types using EAPP (1E4) Mouse mAb. IF-IC
Confocal immunofluorescent analysis of MCF7 cells using EAPP (1E4) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | Regulation of the E2F family of transcription factors, primarily through the retinoblastoma protein (pRb), is vital for control of cell proliferation and cell death (reviewed in 1). E2F-associated phosphoprotein (EAPP) was identified as an E2F-family binding protein that modulates E2F-regulated transcription and may be required for S phase entry. EAPP is expressed at varied levels in all tissues and cell types examined and its expression is reduced in nocodazole-arrested cells (2). Mass spectrometry studies have identified multiple EAPP phosphorylation sites including Ser109 and Ser111, but biological consequences of EAPP phosphorylation have yet to be elucidated (3-5). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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