- 询价
- Cell Signaling Technology
- 2144
- USA
- 2025年09月04日
- W, IHC-P, IF-IC, F
- Rabbit
- H,M,R,Mk,Dm,B,X
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
α-Tubulin Antibody
- 抗原：
synthetic peptide corresponding to the sequence of human α-tubulin
- 应用范围：
W, IHC-P, IF-IC, F
- 宿主：
Rabbit
- 供应商：
CST
- 适应物种：
H,M,R,Mk,Dm,B,X
- 库存：
大量
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Dm=D. melanogaster X=Xenopus B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P IF-IC F | H M R Mk Dm B (X) | Endogenous | 52 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | The α-Tubulin Antibody detects endogenous levels of total α-tubulin protein, and does not cross-react with recombinant β-tubulin. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human α-tubulin. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from CAD and C6 cells, using α-Tubulin Antibody. Western Blotting
Western blot analysis of recombinant α-tubulin and β-tubulin GST-fusion proteins, and extracts from CAD cells, using α-Tubulin Antibody (left), β-Tubulin Antibody #2146 (middle) and GST Antibody #2622 (right). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization using α-Tubulin Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using α-Tubulin Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, using α-Tubulin Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using α-Tubulin Antibody in the presence of control peptide (left) or antigen-specific peptide (right). Flow Cytometry
Flow cytometric analysis of C6 cells, using α-Tubulin Antibody (blue) compared to a nonspecific negative control antibody (red). IF-IC
Confocal immunofluorescent analysis of NIH/3T3 cells, using α-Tubulin Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). |
| Background | The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). |
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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- 作者
- 内容
- 询问日期
文献和实验SDS Gel Electrophoresis of Tubulin\MAPs
. Scan the gels or photographically analyze them. Determine the molecular weights of the proteins (tubulin and MAP's).
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