- 询价
- Cell Signaling Technology
- 3501
- USA
- 2025年10月01日
- western blot,免疫组化（IHC）,免疫荧光（IF）
- 兔
- 人,小鼠,大鼠,驴
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
PDI (C81H6) Rabbit mAb
- 抗原：
/
- 应用范围：
western blot,免疫组化（IHC）,免疫荧光（IF）
- 宿主：
兔
- 库存：
大量
- 供应商：
CST
- 适应物种：
人,小鼠,大鼠,驴
- 级别：
详见MSDS文件
- 抗原来源：
/
- 保质期：
详见说明书
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Translational Control
PDI (C81H6) Rabbit mAb #3501
PhosphoSitePlus ® protein, site, and accession data: PDIA1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC | H M R Mk | Endogenous | 57 | Rabbit |
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
* Product-specific protocol.
Specificity / Sensitivity
PDI (C81H6) Rabbit mAb detects endogenous levels of total PDI protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PDI.
Western Blotting
Western blot analysis of extracts from various cell types using PDI (C81H6) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PDI (C81H6) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lymphoma using PDI (C81H6) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse spleen using PDI (C81H6) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of NIH/3T3 cells using PDI (C81H6) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
During their synthesis, secretory proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Protein disulfide isomerase (PDI) catalyzes the formation and isomerization of these disulfide bonds (2). Studies on mechanisms of oxidative folding suggest that molecular oxygen oxidizes the ER-protein Ero1, which in turn oxidizes PDI through disulfide exchange (3). This event is then followed by PDI-catalyzed disulfide bond formation in folding proteins (3).
- Huppa, J.B. and Ploegh, H.L. (1998) Cell 92, 145-148.
- Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep. 6, 28-32.
- Tu, B.P. and Weissman, J.S. (2004) J. Cell Biol. 164, 341-346.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
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For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验组织化学分析。（图 C）使用Anti-Erk1/2 Mouse mAb 鼠单抗进行目的蛋白检测；(图 D) 使用p44/42 MAPK （Erk1/2）Rabbit mAb 兔单抗进行目的蛋白检测。 图 7 免疫组化实验检测Akt表达 注：使用 Anti-Akt (pan) Rabbit mAb 对正常小鼠肝组织进行免疫组织化学分析。（图 E）使用免疫组化试剂盒 M&R HRP/DAB Detection IHC Kit，抗体1:100 稀释；(图F)使用另一种试剂盒，抗体 1:100 稀释。
h）即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复：简单易行效果好，CST 推荐使用微波炉完成修复。合适的修复液：根据抗体说明书使用合适的修复液。用柠檬酸修复后，切片需浸泡在修复液中，自然冷却；而用 EDTA 修复后，切片可直接从修复缸中取出，直接进行下一步。注：使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb（#4267），EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
到 PVDF 膜上，同时减少蛋白不必要降解，这对于最终获得清晰、可信结果也是需要考虑因素。抗体信息：1.ACC1, Recombinant Rabbit monoclonal IgG. HuaAn. HuaAn biotechnology , inc.2.ATGL, Mouse mAb IgG1, Cat#:RT1058. HuaAn biotechnology , inc.3.p-PERK(Thr981), Rabbit Polyclonal IgG primary antibodies, Cat
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