- 询价
- Cell Signaling Technology
- 3700
- USA
- 2025年12月19日
- W, IHC-P, IF-IC, F
- H,M,R,Hm,Mk
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
β-Actin (8H10D10) Mouse mAb
- 抗原：
synthetic peptide corresponding to amino-terminal residues of human β-actin
- 应用范围：
W, IHC-P, IF-IC, F
- 适应物种：
H,M,R,Hm,Mk
- 库存：
大量
- 保质期：
详见说明书
- 供应商：
CST
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F | H M R Hm Mk | Endogenous | 45 | Mouse IgG2b |
| Protocols |
* Product-specific protocol. |
|---|---|
| Specificity / Sensitivity | β-Actin (8H10D10) Mouse mAb detects endogenous levels of total β-actin protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues of human β-actin. Western Blotting
Western blot analysis of extracts from various cell types using β-Actin (8H10D10) Mouse mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Actin (8H10D10) Mouse mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human heart using β-Actin (8H10D10) Mouse mAb. Note the lack of staining of cardiac muscle. Flow Cytometry
Flow cytometric analysis of HeLa cells using β-Actin (8H10D10) Mouse mAb (blue) compared to a nonspecific negative control antibody (red). IF-IC
Confocal immunofluorescent analysis of NIH/3T3 cells using β-Actin (8H10D10) Mouse mAb (red) and PDI (C81H6) Rabbit mAb #3501 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). IF-IC
Confocal immunofluorescent analysis of A-172 cells using β-Actin (8H10D10) Mouse mAb (green) showing colocalization with actin filaments that have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). Research studies have shown that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis (6).
|
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验常用的β-actin 引物序列 human actin f ctc cat cct ggc ctc gct gt human actin r gct gtc acc ttc acc gtt cc product size:268 rabbit actin r agt gcg acg tgg aca tcc g rabbit actin f tgg ctc taa cag tcc gcc tag product size:295 mouse actin r cgt
human actin f ctc cat cct ggc ctc gct gt human actin r gct gtc acc ttc acc gtt cc product size:268 rabbit actin r agt gcg acg tgg aca tcc g rabbit actin f tgg ctc taa cag tcc gcc ta product size:295 mouse actin r cgt tga cat ccg taa aga
(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
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