- 询价
- Cell Signaling Technology
- 2208S
- USA
- 2025年07月13日
- W IP IF-IC
- H M R Hm Mk
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- 详细信息
- 文献和实验
- 技术资料
- 靶点：
RPA32
- 应用范围：
W IP IF-IC
- 适应物种：
H M R Hm Mk
- 克隆性：
单克隆
- 保存条件：
-20℃
- 规格：
100µl
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文献和实验Immunofluorescent Staining of Mouse and Rat Leukocytes
16 (Mouse BD Fc Block™; Cat No. 553141/553142) or anti-rat CD32 (Rat BD Fc Block™; Cat. No. 550270/550271) is recommended. BD Fc Block™ can be added to cells (~0.25 µg per million cells, 3 - 5 min, 4°C) and need not be washed out prior to addition of primary
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
Dual- and Triple-Color Fluorospot
in the analysis, e.g., IL-10, rat mAb 9D7 is used for coating and tag (different from FITC and biotin)-labeled rat mAb 12G8 is used for detection. All mAbs from Mabtech, Nacka Strand, Sweden. 4. Secondary reagents for amplification: Mouse anti-FITC mAb
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