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- 保存条件:
-20℃
- 供应商:
赛信通(上海)生物试剂有限公司
- 规格:
300µl
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文献和实验nM 5'biotin end-labeled siRNA under gentle agitation, i.e. a rocking plate (Figure 1a). We utilized the EF1 alpha specific siRNA, EF52, shown previously to direct TGS in human cells (Morris et al. 2004). The EF52 siRNA was constructed with a 5' biotin
the vector of choice for siRNA delivery. To this end, we developed a new adenovirus shuttle vector designated as pShuttle-H1 to host H1-RNA promoter and unique Bgl II and Hind III sites for insertion of oligos for expression of siRNA. In this chapter
of tissue/ cell harvest. Dynabeads® mRNA DIRECT™ Kit protocols can be scaled up or down to suit specific sample source and quantity. Please see section "Sample Guidelines and Scaling" before preparing the sample, and for recommended bead and buffer volumes
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