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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
钰博生物
- 检测范围:
见说明书
- 检测方法:
酶联免疫
- 应用:
科研
- 标记物:
见说明书
- 样本:
血清/组织/尿液
| 英文名称 | Rat VEGF-D (Vascular Endothelial Cell Growth Factor D) CLIA Kit | ||
| 中文名称 | 大鼠血管内皮细胞生长因子D(VEGF-D)化学发光免疫检测试剂盒 | ||
| 货号 | YB-R0686c | 种属 | Rat/大鼠 |
| 规格 | 96T/Kit (8*12 strips) 48T/Kit (8*6 strips) | ||
| 检测方法 | 双抗体夹心法 | ||
| 检测范围 | 15.625~1000pg/mL | 灵敏度 | 9.375pg/mL |
| 中文名称 | 英文名称 | 规格 | 保存 |
| CLIA酶标板 | Micro CLIA Plate | 8×12 / 8×6* | 4℃/-20℃ # |
| 冻干标准品 | Reference Standard | 2/1 支* | 4℃/-20℃ # |
| 标准品&样品稀释液 | Reference Standard & Sample Diluent | 1瓶 20mL/12mL* | 4℃ |
| 浓缩生物素化抗体 | Concentrated Biotinylated Detection Ab | 1支 120μL/70μL* | 4℃/-20℃ # |
| 生物素化抗体稀释液 | Biotinytated Detection Ab Diluent | 1瓶 10mL/6mL* | 4℃ |
| 浓缩HRP酶结合物 | Concentrated HRP Conjugate | 1支 120μL/70μL* | 4℃(避光) |
| 酶结合物稀释液 | HRP Conjugate Diluent | 1瓶 10mL/6mL* | 4℃ |
| 浓缩洗涤液(25×) | Concentrated Wash Buffer (25×) | 1瓶 30mL/16mL* | 4℃ |
| 发光底物A液 | Substrate Reagent A | 1瓶 5mL/3mL* | 4℃(避光) |
| 发光底物B液 | Substrate Reagent B | 1瓶 5mL/3mL* | 4℃(避光) |
| 封板覆膜 | Plate Sealer | 5/3 张* | |
| 产品说明书 | Manual | 1 份 | |
| 质检报告 | Certificate of Analysis | 1 份 | |
| Rat VEGF-D (Vascular Endothelial Cell Growth Factor D) CLIA Kit 特别说明: *: [96T/48T](打开包装后请及时检查所有物品是否齐全完整) #: 一周内使用可存于4℃,需长时间存放或多次使用建议存于-20℃. |
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2. 倒去孔内液体,拍干,加入 100μL 生物素化抗体工作液,37℃孵育 60 分钟
3. 洗涤 3 次
4. 加入 100μL 酶结合物工作液, 37℃孵育 30 分钟
5. 洗涤 5 次
6. 加入 100μL 底物溶液, 37℃孵育 5 分钟左右
7. 读数
8. 结果计算
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
. 2. Combining AbC™ anti-Rat/Hamster and ArC™ Amine Reactive Compensation Bead Kits The ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) is designed to facilitate compensation when using any of the LIVE/DEAD® fixable dead cell stains
RNA was purified using the AllPrep DNA/RNA 96 Kit and analyzed on the Agilent 2100 bioanalyzer. Intact RNA is indicated by a RIN value of close to 10. RNA with no genomic DNA contamination. Total RNA was purified from rat kidney
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