- 询价
- R&D
- 美国/中国
- xy-553P
- 2025年07月16日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- Aph-1b/Loop Polyclonal Antibody, Purified保存于运输说明：
- Store at -20°C.
- 详细信息：
-
Description 
Polyclonal Antibody against Aph-1b Loop, O2E2 Form Affinity Purified Antibody (in 1 mg/mL BSA, 0.005% azide, 16.6 % glycerol) Host Rabbit Species Reactivity Human [Ab] Aph-1b/Loop Polyclonal Antibody, Purified1 mg/mL Specificity The gamma secretase is a multiprotein complex consisting of presenilin, nicastrin, Aph-1 and Pen-2; all four proteins are necessary for full proteolytic activity. The gamma secretase complex catalyzes cleavage of a number of integral membrane proteins including Amyloid precursor protein, Notch, Erb4 and E-cadherin. While Aph-1 stabilizes the presenilin holoprotein within the gamma secretase complex, Pen-2 is required for endoproteolysis of presenilin and activation of the gamma secretase.
The Aph-1 homologue on human chromosome 1 is termed Aph-1a and on human chromosome 15 is referred to as Aph-1b.The antibody Aph-1b/Loop(O2E2) was raised against the human Aph-1b/loop region and recognizes the sequence CKLLKKASEGLKSINPGETAPSMR of human Aph-1b. It was subsequently peptide affintiy purified.
Covance also offers an antibody O2D1 (PRB-552P) which recognizes the Aph-1b/C-terminal region.Uses This antibody is effective in immunoblotting and immunostaining. Suggested Working Dilution Aph-1b/Loop Polyclonal Antibody, PurifiedThe optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. - Western blot: 1:1,000
- Immunostaining: 1:1,000
Storage Store at -20°C. It is suggested that the total volume be divided into useable aliquots upon initial thaw. Stable for more than one year. Avoid multiple freeze thaw cycles. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - Aph-1b/Loop Polyclonal Antibody, Purified
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文献和实验Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇：make an Antibody against a Peptides 下一篇：Immunization Protocol for Monoclonal Antibody Production
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
Construction of Polyclonal Antibody Libraries Using Phage Display
Polyclonal antibody libraries (PCALs) are standardized mixtures of antibodies (Abs) specific for an antigen (Ag) or multi-Ag target (a poly-Ag). As the immunoglobulin (Ig) genes are cloned, the mixtures can be perpetuated, amplified
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