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- 详细信息
- 文献和实验
- 技术资料
- 形态:
液态/粉状
- 保存条件:
-20℃保存
- 克隆性:
多克隆
- 标记物:
见说明书
- 适应物种:
人,大鼠,小鼠,兔
- 宿主:
Goat,Rabbit,Mouse
- 应用范围:
科研使用
- 浓度:
1mg/1ml
- 靶点:
来电咨询
- MAP2 Polyclonal Antibody, Purified保存于运输说明:
- Store at -20°C
- 详细信息:
-
Description 
Polyclonal Antibody against Microtubule Associated Protein 2 Form 
Purified Antibody Host 
Chicken Species Reactivity 
Human, Rat, Mouse, Bovine IsoType 
IgY [Ab] 
MAP2 Polyclonal Antibody, Purified~19mg/mL Specificity 
Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one α subunit and one β tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein τ (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein τ (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast τ (tau) is found predominantly in neuronal axons.
This antibody was made against MAP2 in chickens.Uses 
MAP2 Polyclonal Antibody, PurifiedThis antibody is effective in immunoblotting (WB) and immunofluorescence (IF). This antibody does not bind to protein A or protein G. Suggested Working Dilution 
The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. - WB: 1:100,000*
- IF: 1:10,000
Notes 
*Predicted MW = 280kD and a doublet ~70kD Storage 
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Warranty/Conditions 
Covance products may not be resold or modified for resale without prior written approval. - MAP2 Polyclonal Antibody, Purified
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文献和实验Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇:make an Antibody against a Peptides 下一篇:Immunization Protocol for Monoclonal Antibody Production
Polyclonal Antibody Production
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Construction of Polyclonal Antibody Libraries Using Phage Display
Polyclonal antibody libraries (PCALs) are standardized mixtures of antibodies (Abs) specific for an antigen (Ag) or multi-Ag target (a poly-Ag). As the immunoglobulin (Ig) genes are cloned, the mixtures can be perpetuated, amplified
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