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- 详细信息
- 文献和实验
- 技术资料
- 形态:
液态/粉状
- 保存条件:
-20℃保存
- 克隆性:
多克隆
- 标记物:
见说明书
- 适应物种:
人,大鼠,小鼠,兔
- 宿主:
Goat,Rabbit,Mouse
- 应用范围:
科研使用
- 浓度:
1mg/1ml
- 靶点:
来电咨询
- MAP2 Monoclonal Antibody, Purified保存于运输说明:
- Store at -20°C
- 详细信息:
-
Description 
Monoclonal Antibody against MAP2 Intended Use 
**Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Clone 
AA6 Form 
Purified Antibody (in PBS + 0.03% thimerosal) Host 
MAP2 Monoclonal Antibody, PurifiedMouse Species Reactivity 
Mammalian IsoType 
IgG2a [Ab] 
1 mg/mL Specificity 
Microtubules are required for many well characterized functions in eukaryotic cells, including the movement of chromosomes in mitosis and meiosis, intracellular transport, establishment and maintenance of cellular morphology, cell growth, cell migration and morphogenesis in multicellular organisms. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription. This antibody will stain neuronal cell bodies and dendritic processes, and will not stain glial cells or axons. It is the best marker to distinguish dendrites from axons in the brain. Uses 
MAP2 Monoclonal Antibody, PurifiedThis antibody is effective in immunoblotting. Suggested Working Dilution 
The optimal working dilution should be determined for each specific assay condition. - Western blot: 1:1,000*
Notes 
*Predicted MW = 280kDa Storage 
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. References 
Olmsted JB. Microtubule-associated proteins. Annu Rev Cell Biol 2:421-57, 1986.
Matus A. Microtubule associated proteins: Their potential role in determining neuronal morphology. Annu Rev Neurosci 11:29-44, 1988.Warranty/Conditions 
Covance products may not be resold or modified for resale without prior written approval. - MAP2 Monoclonal Antibody, Purified
- xy-0369M-xyE-xyy5.5 Mouse Anti-Rabbit IgM/PE-Cy5.5PE-Cy5.5标记的小鼠抗兔IgM
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xy-0374R-Golxy Rabbit Anti-horse IgM/Gold胶体金标记的兔抗马IgM
xy-0374R-AF647 Rabbit Anti-horse IgM/Alexa Fluor 647Alexa Fluor 647标记的兔抗马IgM
xy-0374R-Axyxy Rabbit Anti-horse IgM/APCAPC标记的兔抗马IgM
xy-0374R-AF350 Rabbit Anti-horse IgM/Alexa Fluor 350Alexa Fluor 350标记的兔抗马IgM
xy-0374R-AF488 Rabbit Anti-horse IgM/Alexa Fluor 488Alexa Fluor 488标记的兔抗马IgM
xy-0374R-AF555 Rabbit Anti-horse IgM/Alexa Fluor 555Alexa Fluor 555标记的兔抗马IgM
xy-0374R-RBITxy Rabbit Anti-horse IgM/RBITC罗丹明标记的兔抗马IgM
xy-0374R-xyy3 Rabbit Anti-horse IgM/Cy3Cy3标记的兔抗马IgM
xy-0374R-xyy5.5 Rabbit Anti-horse IgM/Cy5.5Cy5.5标记的兔抗马IgM
xy-0374R-xyy5 Rabbit Anti-horse IgM/Cy5Cy5标记的兔抗马IgM
xy-0374R-xyy7 Rabbit Anti-horse IgM/Cy7Cy7标记的兔抗马IgM
xy-0374R-xyE Rabbit Anti-horse IgM/PEPE标记的兔抗马IgM
xy-0374R-FITxy Rabbit Anti-horse IgM/FITCFITC标记的兔抗马IgM
xy-0374R-Bio Rabbit Anti-horse IgM/Bio生物素标记的兔抗马IgM
xy-0326G-xyy5 Goat Anti-Bovine IgG/Cy5Cy5标记的羊抗牛IgG
xy-0326G-Bio Goat Anti-Bovine IgG/Bio生物素标记的羊抗牛IgG
xy-0326G-FITxy Goat Anti-Bovine IgG/FITCFITC标记的羊抗牛IgG
xy-0326G-RBITxy Goat Anti-Bovine IgG/RBITC罗丹明标记的羊抗牛IgG
xy-0326G-xyE-xyy3 Goat Anti-Bovine IgG/PE-Cy3PE-Cy3标记的羊抗牛IgG
xy-0326G-xyE-xyY5 Goat Anti-Bovine IgG/PE-CY5PE-CY5标记的羊抗牛IgG
xy-0326G-Axyxy Goat Anti-Bovine IgG/APCAPC标记的羊抗牛IgG
xy-0326G-AF350 Goat Anti-Bovine IgG/Alexa Fluor 350Alexa Fluor 350标记的羊抗牛IgG
xy-0326G-AF488 Goat Anti-Bovine IgG/Alexa Fluor 488Alexa Fluor 488标记的羊抗牛IgG
xy-0326G-AF555 Goat Anti-Bovine IgG/Alexa Fluor 555Alexa Fluor 555标记的羊抗牛IgG
xy-0326G-AF647 Goat Anti-Bovine IgG/Alexa Fluor 647Alexa Fluor 647标记的羊抗牛IgG
xy-0326G-xyE-xyy5.5 Goat Anti-Bovine IgG/PE-Cy5.5PE-Cy5.5标记的羊抗牛IgG
xy-0326G-xyE-xyy7 Goat Anti-Bovine IgG/PE-Cy7PE-Cy7标记的羊抗牛IgG
xy-0326G-xyy7 Goat Anti-Bovine IgG/Cy7Cy7标记的羊抗牛IgG
xy-0326G-Axy Goat Anti-Bovine IgG/AP碱性磷酸酶(AP)标记的羊抗牛IgG
xy-0326G-Golxy Goat Anti-Bovine IgG/Gold胶体金标记的羊抗牛IgG
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文献和实验指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma,在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应,对应于抗原之不同部分可制成各种不同的抗体复合体。然而,因为一个产生抗体的细胞,只能产生一种抗体,所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种,可形成边产生抗体边增殖的 hybridoma,将每个杂种细胞进行克隆(无性繁殖系)培养,则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成,这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Affinity Chromatography
all the above criteria and also afford the advantage of being available in essentially unlimited supply. Moreover, they are themselves readily purified, often by affinity chromatography. The main disadvantage of monoclonal antibody ligands is that they must possess
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
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