Beckman A63881现货Agencourt AMPu

Beckman货号A63881现货19121878899上海睿安生物

AMPure XP是一种能够获得高质量DNA产物的纯化试剂盒，纯化后的核酸纯度高，无盐离子残留，无需离心或过滤，目前广泛应用于NGS文库构建。该试剂的应用为产物纯化，如：NGS文库构建过程中酶联体系的纯化、PCR产物纯化等。AMPure XP试剂相应的货号如下：

Application: PCR purification

Downstream Application Techniques: PCR, genotyping (SNP detection) fragment analysis, sequencing (Sanger and Next Generation), cloning, primer walking

• High recovery of amplicons, greater than 100 bp
• Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
• Stable PCR products post-cleanup: No PCR degradation after storage at 4° C for seven days
• Efficient recovery of double stranded and single stranded DNA templates
• Consistent recovery throughout the kit’s 12 month shelf life
• Faster manual and automated processing as compared to traditional post-PCR cleanup methods

1. PCR reaction 2. Binding of PCR amplicons to magnetic beads 3. Separation of PCR amplicons bound to magnetic beads from contaminants 4. Washing of PCR amplicons with Ethanol 5. Elution of PCR amplicons from the magnetic particles 6. Transfer away from the beads into a new plate

Agencourt AMPure XP purification system recovers amplicons greater than 100 bp with consistency and high reproducibility. The recovery efficiency of the Agencourt AMPure XP kit for small and large amplicon sizes is superior to the traditional filtration PCR cleanup methods (Figure 1).

Figure 1. The recovery of the PCR products post-PCR cleanup was measured by spectrophotometry; 200 bp, 800 bp and 10 Kb PCR products where purified with the Agencourt AMPure XP kit, QIAquick* kit and QuickStep* filtration system.