- 询价
- R&D
- 美国/中国
- xy-3473
- 2025年07月13日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- Neurofilament (2F11) Monoclonal Antibody (SIGNET)详细信息：
-
Description 
Monoclonal Antibody against Neurofilament (NF)
(Formerly Signet Catalog Nos: 473-01, 473-16 L1 Predilute, 473-26 L2 Predilute)Intended Use ** In Vitro Diagnostic (IVD) ** Clone 2F11 Form Tissue Culture Supernatant (in 0.01M PBS + 0.1% NaN3 + 1% BSA) Host Neurofilament (2F11) Monoclonal Antibody (SIGNET)Mouse IsoType IgG1 Specificity The Signet monoclonal neurofilament antibody clone 2F11 reacts with the 70kDa subunit of neurofilament and stains neurons in the central and peripheral nervous systems. Neuroblastomas and ganglioneuromas stain positively with this antibody, as do a proportion of pheochromocytomas and esthesioblastomas.
This antibody was developed using human brain neurofilament.
Concentrated Format:
SIG-3473-1000 [1 mL]
Prediluted Formats:
SIG-3473-16 [6 mL] is ready to use with Peroxidase Anti- Peroxidase detection systems such as Signet’s PAP (SIG-32140).
SIG-3473-26 [6 mL] is ready-to-use with Biotin-based detection systems such as Signet’s USA Ultrastreptavidin Detection (SIG-32250).Uses Neurofilament (2F11) Monoclonal Antibody (SIGNET)This antibody is effective in immunohistochemistry (IHC). Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as Signet’s USA Ultrastreptavidin Detection (SIG-32250).
Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: Not required
Incubation: 20 minutes at room temperature
Notes Positive tissue (human): Cerebellum Storage Store between 2-8°C. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as Signet’s USA Ultrastreptavidin Detection (SIG-32250).
- Neurofilament (2F11) Monoclonal Antibody (SIGNET)
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文献和实验免疫组化技术-Immunohistochemical Protocols
. FITC-tyramide (green) is used in a simple fluorescent amplification (path 12) for the first antigen, glucagon, while Cy3-conjugated secondary antibody (red) is used in the conventional immunostaining (path 1) for the second antigen, neurofilament
gravis 重症肌无力 β-MG β-microglobin β-微球蛋白 MBP myelic basic protein 髓磷酯碱性蛋白 MBPAb myelic basic protein antibody 髓磷酯碱性蛋白抗体 McAB monoclonal antibody 单克隆抗体 MF microfilament 微丝 Mg myohemoglobin 肌红蛋白 MHC man histocompatibility
In Vitro Analysis of Mouse Mesencephalic Neural Crest Development
crest development. Dev. Biol. 157:517‐525. Kubota, Y., Morita, T., and Ito, K. 1996. New monoclonal antibody (4E9R) identifies mouse neural crest cells. Dev. Dyn. 206:368‐378
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