- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YBEA143Ca
- 2025年07月16日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Canis familiaris; Canine (Dog，犬)
血管内皮生长因子A(VEGFA)检测试剂盒
检测范围 15.63-1000pg/mL 灵敏度 6.5pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
血管内皮生长因子A(VEGFA)检测试剂盒
ybA012Ra 大鼠S100钙结合蛋白(S100)ELISA试剂盒 ELISA Kit for S100 Calcium Binding Protein (S100)
ybA413Ra 大鼠粘蛋白1(MUC1)ELISA试剂盒 ELISA Kit for Mucin 1 (MUC1)
ybA019Ra 大鼠白介素8受体α(IL8Rα)ELISA试剂盒 ELISA Kit for Interleukin 8 ReHZptor Alpha (IL8Ra)
ybA431Ra 大鼠颗粒酶M(GZMM)ELISA试剂盒 ELISA Kit for Granzyme M (GZMM)
ybA235Ra 大鼠补体1抑制因子(C1INH)ELISA试剂盒 ELISA Kit for Complement 1 Inhibitor (C1INH)
ybC488Ra 大鼠亚铁螯合酶(FECH)ELISA试剂盒 ELISA Kit for FerrochelataHZ (FECH)
ybB067Ra 大鼠YY肽(PYY)ELISA试剂盒 ELISA Kit for Peptide YY (PYY)
ybC864Ra 大鼠硬骨素(SOST)ELISA试剂盒 ELISA Kit for Sclerostin (SOST)
ybC418Ra 大鼠过氧化氢酶(CAT)ELISA试剂盒 ELISA Kit for CatalaHZ (CAT)
ybC992Ra 大鼠血浆谷胱甘肽过氧化物酶(GPX3)ELISA试剂盒 ELISA Kit for Glutathione PeroxidaHZ 3, Plasma (GPX3)
ybB447Ra 大鼠乙酰胆碱酯酶(AchE)ELISA试剂盒 ELISA Kit for AHZtylcholinesteraHZ (AchE)
ybA542Ra 大鼠内皮抑素(ES)ELISA试剂盒 ELISA Kit for Endostatin (ES)
ybB254Ra 大鼠血管抑素(ANG)ELISA试剂盒 ELISA Kit for Angiostatin (ANG)
ybB653Ra 大鼠肌肉生长抑制素(MSTN)ELISA试剂盒 ELISA Kit for Myostatin (MSTN)
ybA527Ra 大鼠氧化低密度脂蛋白(OxLDL)ELISA试剂盒 ELISA Kit for Oxidized Low Density Lipoprotein (OxLDL)
ybA242Ra 大鼠摄食抑制因子1(NES1)ELISA试剂盒 ELISA Kit for Nesfatin 1 (NES1)
ybA608Ra 大鼠微粒体谷胱甘肽S转移酶1(MGST1)ELISA试剂盒 ELISA Kit for Microsomal Glutathione S TransferaHZ 1 (MGST1)
ybB939Ra 大鼠多配体蛋白聚糖4(SDC4)ELISA试剂盒 ELISA Kit for Syndecan 4 (SDC4)
ybB477Ra 大鼠基质Gla蛋白(MGP)ELISA试剂盒 ELISA Kit for Matrix Gla Protein (MGP)
ybA048Ra 大鼠抑制素βE(INHβE)ELISA试剂盒 ELISA Kit for Inhibin Beta E (INHbE)
ybA672Ra 大鼠Slit同源物2(Slit2)ELISA试剂盒 ELISA Kit for Slit Homolog 2 (Slit2)
ybA613Ra 大鼠谷胱甘肽S转移酶θ2(GSTθ2)ELISA试剂盒 ELISA Kit for Glutathione S TransferaHZ Theta 2 (GSTt2)
ybA406Ra 大鼠性别决定区Y框蛋白2(SOX2)ELISA试剂盒 ELISA Kit for HZx Determining Region Y Box Protein 2 (SOX2)
ybA424Ra 大鼠八聚体结合转录因子4(OCT4)ELISA试剂盒 ELISA Kit for Octamer Binding Transcription Factor 4 (OCT4)
ybB241Ra 大鼠Kruppel样因子4(KLF4)ELISA试剂盒 ELISA Kit for Kruppel Like Factor 4, Gut (KLF4)
血管内皮生长因子A(VEGFA)检测试剂盒
检测范围 15.63-1000pg/mL 灵敏度 6.5pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
血管内皮生长因子A(VEGFA)检测试剂盒
ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Canis familiaris; Canine (Dog) |
| Product No. | SEA143Ca |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 15.63-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 6.5pg/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Factor A (VEGFA).
No significant cross-reactivity or interference between Vascular Endothelial Growth Factor A (VEGFA) and analogues was observed.
No significant cross-reactivity or interference between Vascular Endothelial Growth Factor A (VEGFA) and analogues was observed.
Recovery
血管内皮生长因子A(VEGFA)检测试剂盒Matrices listed below were spiked with certain level of recombinant Vascular Endothelial Growth Factor A (VEGFA) and the recovery rates were calculated by comparing the measured value to the expected amount of Vascular Endothelial Growth Factor A (VEGFA) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 88-95 | 92 |
| EDTA plasma(n=5) | 83-93 | 88 |
| heparin plasma(n=5) | 88-102 | 91 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Endothelial Growth Factor A (VEGFA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Endothelial Growth Factor A (VEGFA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Endothelial Growth Factor A (VEGFA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vascular Endothelial Growth Factor A (VEGFA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-102% | 79-103% | 94-103% | 79-97% |
| EDTA plasma(n=5) | 89-98% | 93-104% | 90-98% | 83-98% |
| heparin plasma(n=5) | 84-91% | 78-88% | 78-98% | 87-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
血管内皮生长因子A(VEGFA)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Factor A (VEGFA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vascular Endothelial Growth Factor A (VEGFA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vascular Endothelial Growth Factor A (VEGFA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vascular Endothelial Growth Factor A (VEGFA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ybB224Ra 大鼠胃泌素(GT)ELISA试剂盒 ELISA Kit for Gastrin (GT)
ybA012Ra 大鼠S100钙结合蛋白(S100)ELISA试剂盒 ELISA Kit for S100 Calcium Binding Protein (S100)
ybA413Ra 大鼠粘蛋白1(MUC1)ELISA试剂盒 ELISA Kit for Mucin 1 (MUC1)
ybA019Ra 大鼠白介素8受体α(IL8Rα)ELISA试剂盒 ELISA Kit for Interleukin 8 ReHZptor Alpha (IL8Ra)
ybA431Ra 大鼠颗粒酶M(GZMM)ELISA试剂盒 ELISA Kit for Granzyme M (GZMM)
ybA235Ra 大鼠补体1抑制因子(C1INH)ELISA试剂盒 ELISA Kit for Complement 1 Inhibitor (C1INH)
ybC488Ra 大鼠亚铁螯合酶(FECH)ELISA试剂盒 ELISA Kit for FerrochelataHZ (FECH)
ybB067Ra 大鼠YY肽(PYY)ELISA试剂盒 ELISA Kit for Peptide YY (PYY)
ybC864Ra 大鼠硬骨素(SOST)ELISA试剂盒 ELISA Kit for Sclerostin (SOST)
ybC418Ra 大鼠过氧化氢酶(CAT)ELISA试剂盒 ELISA Kit for CatalaHZ (CAT)
ybC992Ra 大鼠血浆谷胱甘肽过氧化物酶(GPX3)ELISA试剂盒 ELISA Kit for Glutathione PeroxidaHZ 3, Plasma (GPX3)
ybB447Ra 大鼠乙酰胆碱酯酶(AchE)ELISA试剂盒 ELISA Kit for AHZtylcholinesteraHZ (AchE)
ybA542Ra 大鼠内皮抑素(ES)ELISA试剂盒 ELISA Kit for Endostatin (ES)
ybB254Ra 大鼠血管抑素(ANG)ELISA试剂盒 ELISA Kit for Angiostatin (ANG)
ybB653Ra 大鼠肌肉生长抑制素(MSTN)ELISA试剂盒 ELISA Kit for Myostatin (MSTN)
ybA527Ra 大鼠氧化低密度脂蛋白(OxLDL)ELISA试剂盒 ELISA Kit for Oxidized Low Density Lipoprotein (OxLDL)
ybA242Ra 大鼠摄食抑制因子1(NES1)ELISA试剂盒 ELISA Kit for Nesfatin 1 (NES1)
ybA608Ra 大鼠微粒体谷胱甘肽S转移酶1(MGST1)ELISA试剂盒 ELISA Kit for Microsomal Glutathione S TransferaHZ 1 (MGST1)
ybB939Ra 大鼠多配体蛋白聚糖4(SDC4)ELISA试剂盒 ELISA Kit for Syndecan 4 (SDC4)
ybB477Ra 大鼠基质Gla蛋白(MGP)ELISA试剂盒 ELISA Kit for Matrix Gla Protein (MGP)
ybA048Ra 大鼠抑制素βE(INHβE)ELISA试剂盒 ELISA Kit for Inhibin Beta E (INHbE)
ybA672Ra 大鼠Slit同源物2(Slit2)ELISA试剂盒 ELISA Kit for Slit Homolog 2 (Slit2)
ybA613Ra 大鼠谷胱甘肽S转移酶θ2(GSTθ2)ELISA试剂盒 ELISA Kit for Glutathione S TransferaHZ Theta 2 (GSTt2)
ybA406Ra 大鼠性别决定区Y框蛋白2(SOX2)ELISA试剂盒 ELISA Kit for HZx Determining Region Y Box Protein 2 (SOX2)
ybA424Ra 大鼠八聚体结合转录因子4(OCT4)ELISA试剂盒 ELISA Kit for Octamer Binding Transcription Factor 4 (OCT4)
ybB241Ra 大鼠Kruppel样因子4(KLF4)ELISA试剂盒 ELISA Kit for Kruppel Like Factor 4, Gut (KLF4)
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文献和实验相关实验
9.12 Cell Res：VEGFA 信号通路在血管稳态调控过程中的两面性
① Cell Res：VEGFA 信号通路在血管稳态调控过程中的两面性内皮细胞响应信号刺激对血管形态、稳态和功能的调控至关重要。血管内皮生长因子 A (VEGFA) 自 20 世纪 80 年代被鉴定以来，其主要功能之一便是促进血管生长。中国科学院上海营养与健康研究所潘巍峻研究组在斑马鱼中发现，在 VEGFA 刺激条件下，cds2 基因突变胚胎的血管能够发生血管退化。斑马鱼活体成像技术表明，cds2 突变体的内皮细胞受 VEGFA 刺激能够通过逆向迁移和细胞凋亡来诱导血管退化。小鼠
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因子）提供预选型的 HUVEC.这些细胞都经过 PromoCell 独有的 3D 血管生成检测试剂盒检测验证为阳性。VEGF 是一种重要的信号传导蛋白质，在血管发生和血管形成的过程中均有涉及。在活体外，研究显示 VEGF 可以刺激内皮细胞有丝分裂发生、细胞移行、血管出芽和微血管渗透。The cells have been tested: Von Willebrand factor （vWF） positive, CD31 positive, Dil-Ac-LDL uptake positive
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血管内皮生长因子A(VEGFA)检测试剂盒
¥900 - 1890
