- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YBEA031Mu
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Mus musculus (Mouse，小鼠)
凋亡相关因子配体(FASL)检测试剂盒
检测范围 0.156-10ng/mL 灵敏度 0.061ng/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
凋亡相关因子配体(FASL)检测试剂盒
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凋亡相关因子配体(FASL)检测试剂盒
检测范围 0.156-10ng/mL 灵敏度 0.061ng/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
凋亡相关因子配体(FASL)检测试剂盒
ELISA Kit for Factor Related Apoptosis Ligand (FASL)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Mus musculus (Mouse) |
| Product No. | SEA031Mu |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 0.061ng/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Factor Related Apoptosis Ligand (FASL).
No significant cross-reactivity or interference between Factor Related Apoptosis Ligand (FASL) and analogues was observed.
No significant cross-reactivity or interference between Factor Related Apoptosis Ligand (FASL) and analogues was observed.
Recovery
凋亡相关因子配体(FASL)检测试剂盒Matrices listed below were spiked with certain level of recombinant Factor Related Apoptosis Ligand (FASL) and the recovery rates were calculated by comparing the measured value to the expected amount of Factor Related Apoptosis Ligand (FASL) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 80-90 | 83 |
| EDTA plasma(n=5) | 79-91 | 85 |
| heparin plasma(n=5) | 85-93 | 90 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Factor Related Apoptosis Ligand (FASL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Factor Related Apoptosis Ligand (FASL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Factor Related Apoptosis Ligand (FASL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Factor Related Apoptosis Ligand (FASL) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-93% | 94-102% | 80-101% | 79-96% |
| EDTA plasma(n=5) | 81-96% | 86-101% | 87-104% | 94-102% |
| heparin plasma(n=5) | 95-102% | 84-103% | 90-101% | 80-89% |
Stability
凋亡相关因子配体(FASL)检测试剂盒The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Factor Related Apoptosis Ligand (FASL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Factor Related Apoptosis Ligand (FASL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Factor Related Apoptosis Ligand (FASL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Factor Related Apoptosis Ligand (FASL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验相关实验
大鼠凋亡相关因子配体(FASL) 酶联免疫分析（ELISA ） 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定大鼠血清，细胞上清及相关液体样本中凋亡相关因子配体(FASL) 的 含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中大鼠凋亡相关因子配体 (FASL) 水平。用纯化的大鼠凋亡相关因子配体 (FASL) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入凋亡相关因子配体
，从而诱导 T 细胞凋亡。PMID: 15655551②来源于肿瘤细胞的外泌体能携带 TRAIL、galectin 9 或 FASL 分子， 从而诱导 CD8 +T 细胞的凋亡，如黑素瘤 TEXs 表达 FasL 在体内能潜在地诱导 T 细胞凋亡。PMID: 19005181③FrarIgsmyr 等发现合胞体 (胚胎) 滋养层能产生 FasL 并以 exosome 的形式释放到胞外，这些胚胎中含 FasL 的 exosome 诱导表达 Fas 的效应细胞凋亡。PMID: 15579659④膀胱
还是不全面的，目前比较清楚的通路 主要有： 1)细胞凋亡的膜受体通路：各种外界因素是细胞凋亡的启动剂，它们可以通过不同的信号传递系统传递凋亡信号，引起细胞凋亡，我们以Fas -FasL为例： Fas是一种跨膜蛋白，属于肿瘤坏死因子受体超家族成员，它与FasL结合可以启动凋亡信号的转导引起细胞凋亡。它的活化包括一系列步骤：首先配体诱导受体三聚体化，然后在细胞膜 上形成凋亡诱导复合物，这个复合物中包括带有死亡结构域的Fas相关蛋白FADD。Fas又称CD95，是由325
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凋亡相关因子配体(FASL)检测试剂盒
¥900 - 1890
