- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YBEA011Hu
- 2026年05月29日
14 年钻石会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
源叶生物 十万种现货试剂速发，品质稳、品类全，实验不再等！Takara 秋促来袭，提取+反转录+定量套装仅 1200 元abinScience 体内级抗体 全年锁价 低至 ￥1200阿拉丁 22万+ 科研级产品现货速发，品质严控、科研无忧- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Homo sapiens (Human，人)
脑源性神经营养因子(BDNF)检测试剂盒
检测范围 0.156-10ng/mL 灵敏度 0.068ng/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
脑源性神经营养因子(BDNF)检测试剂盒
yb-E11951 小鼠高敏三碘甲状腺原氨酸(u-T3)ELISA试剂盒 Three high-sensitivity mouse triiodothyronine (u-T3) ELISA kit
yb-E11952 小鼠B细胞淋巴瘤因子2(Bcl-2)ELISA试剂盒 Murine B cell lymphoma factor 2 (Bcl-2) ELISA Kit
yb-E11953 小鼠CXC趋化因子受体3(CXCR3)ELISA试剂盒 Mouse CXC chemokine receptor 3 (CXCR3) ELISA kit
yb-E11954 小鼠肿瘤特异生长因子/肿瘤相关因子(TGSF)ELISA试剂盒 Mouse tumor specific growth factor / tumor-associated factor (TGSF) ELISA kit
yb-E11955 小鼠羟脯氨酸(Hyp)ELISA试剂盒 Mice hydroxyproline (Hyp) ELISA Kit
yb-E11956 小鼠骨保护素配体(OPGL)ELISA试剂盒 Mice osteoprotegerin ligand (OPGL) ELISA kit
yb-E11957 小鼠乳铁传递蛋白/乳铁蛋白(LF/LTF)ELISA试剂盒 Mouse lactoferrin / lactoferrin (LF / LTF) ELISA Kit
yb-E11958 小鼠抗肌内膜抗体IgA(EMA IgA)ELISA试剂盒 Mouse anti- endomysial antibody IgA (EMA IgA) ELISA kit
yb-E11959 小鼠铁蛋白(FE)ELISA试剂盒 Mouse ferritin (FE) ELISA Kit
yb-E11960 小鼠碳酸酐酶2(CA-2)ELISA试剂盒 Mouse carbonic anhydrase 2 (CA-2) ELISA Kit
yb-E11961 小鼠色素上皮衍生因子(PEDF)ELISA试剂盒 Mice pigment epithelium-derived factor (PEDF) ELISA kit
yb-E11962 小鼠解整合素样金属蛋白酶8(ADAM8)ELISA试剂盒 Mice disintegrin -like metalloproteinase 8 (ADAM8) ELISA kit
yb-E11963 小鼠抗胰蛋白酶(AT)ELISA试剂盒 Mice antitrypsin (AT) ELISA Kit
yb-E11964 小鼠胰岛细胞抗体(ICA)ELISA试剂盒 Mouse islet cell antibodies (ICA) ELISA kit
yb-E11965 小鼠肾损伤分子1(Kim-1)ELISA试剂盒 Mouse kidney injury molecule 1 (Kim-1) ELISA Kit
yb-E11966 小鼠抗单核细胞抗体(AMA)ELISA试剂盒 Mouse anti- monocyte antibodies (AMA) ELISA Kit
yb-E11967 小鼠氧化低密度脂蛋白抗体(OLAb)ELISA试剂盒 LDL oxidation mouse antibody (OLAb) ELISA Kit
yb-E11968 小鼠抗平滑肌抗体(ASMA)ELISA试剂盒 Mouse anti-smooth muscle antibodies (ASMA) ELISA Kit
yb-E11969 小鼠胚胎干细胞系MESPU30(M30)ELISA试剂盒 Mouse embryonic stem cell lines MESPU30 (M30) ELISA kit
yb-E11970 小鼠核因子κB亚基p65亲和肽(NF-κB p65)ELISA试剂盒 Mice nuclear factor κB p65 subunit affinity peptide (NF-κB p65) ELISA kit
yb-E11971 小鼠血小板膜糖蛋白ⅡbⅢa(GP-ⅡbⅢa/CD41+CD61)ELISA试剂盒 Mouse platelet membrane glycoprotein Ⅱ b Ⅲ a (GP-Ⅱ b Ⅲ a/CD41 + CD61) ELISA Kit
yb-E11972 小鼠游离脂肪酸(FFA)ELISA试剂盒 Mice free fatty acid (FFA) ELISA Kit
yb-E11973 小鼠血小板反应蛋白/凝血酶敏感蛋白1(TSP-1)ELISA试剂盒 Mouse platelet -reactive protein / thrombospondin 1 (TSP-1) ELISA Kit
脑源性神经营养因子(BDNF)检测试剂盒
检测范围 0.156-10ng/mL 灵敏度 0.068ng/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
脑源性神经营养因子(BDNF)检测试剂盒
ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | SEA011Hu |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 0.068ng/mL. |
Specificity
脑源性神经营养因子(BDNF)检测试剂盒This assay has high sensitivity and excellent specificity for detection of Brain Derived Neurotrophic Factor (BDNF).
No significant cross-reactivity or interference between Brain Derived Neurotrophic Factor (BDNF) and analogues was observed.
No significant cross-reactivity or interference between Brain Derived Neurotrophic Factor (BDNF) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Brain Derived Neurotrophic Factor (BDNF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Brain Derived Neurotrophic Factor (BDNF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Brain Derived Neurotrophic Factor (BDNF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
脑源性神经营养因子(BDNF)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Brain Derived Neurotrophic Factor (BDNF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Brain Derived Neurotrophic Factor (BDNF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Brain Derived Neurotrophic Factor (BDNF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Brain Derived Neurotrophic Factor (BDNF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
yb-E11951 小鼠高敏三碘甲状腺原氨酸(u-T3)ELISA试剂盒 Three high-sensitivity mouse triiodothyronine (u-T3) ELISA kit
yb-E11952 小鼠B细胞淋巴瘤因子2(Bcl-2)ELISA试剂盒 Murine B cell lymphoma factor 2 (Bcl-2) ELISA Kit
yb-E11953 小鼠CXC趋化因子受体3(CXCR3)ELISA试剂盒 Mouse CXC chemokine receptor 3 (CXCR3) ELISA kit
yb-E11954 小鼠肿瘤特异生长因子/肿瘤相关因子(TGSF)ELISA试剂盒 Mouse tumor specific growth factor / tumor-associated factor (TGSF) ELISA kit
yb-E11955 小鼠羟脯氨酸(Hyp)ELISA试剂盒 Mice hydroxyproline (Hyp) ELISA Kit
yb-E11956 小鼠骨保护素配体(OPGL)ELISA试剂盒 Mice osteoprotegerin ligand (OPGL) ELISA kit
yb-E11957 小鼠乳铁传递蛋白/乳铁蛋白(LF/LTF)ELISA试剂盒 Mouse lactoferrin / lactoferrin (LF / LTF) ELISA Kit
yb-E11958 小鼠抗肌内膜抗体IgA(EMA IgA)ELISA试剂盒 Mouse anti- endomysial antibody IgA (EMA IgA) ELISA kit
yb-E11959 小鼠铁蛋白(FE)ELISA试剂盒 Mouse ferritin (FE) ELISA Kit
yb-E11960 小鼠碳酸酐酶2(CA-2)ELISA试剂盒 Mouse carbonic anhydrase 2 (CA-2) ELISA Kit
yb-E11961 小鼠色素上皮衍生因子(PEDF)ELISA试剂盒 Mice pigment epithelium-derived factor (PEDF) ELISA kit
yb-E11962 小鼠解整合素样金属蛋白酶8(ADAM8)ELISA试剂盒 Mice disintegrin -like metalloproteinase 8 (ADAM8) ELISA kit
yb-E11963 小鼠抗胰蛋白酶(AT)ELISA试剂盒 Mice antitrypsin (AT) ELISA Kit
yb-E11964 小鼠胰岛细胞抗体(ICA)ELISA试剂盒 Mouse islet cell antibodies (ICA) ELISA kit
yb-E11965 小鼠肾损伤分子1(Kim-1)ELISA试剂盒 Mouse kidney injury molecule 1 (Kim-1) ELISA Kit
yb-E11966 小鼠抗单核细胞抗体(AMA)ELISA试剂盒 Mouse anti- monocyte antibodies (AMA) ELISA Kit
yb-E11967 小鼠氧化低密度脂蛋白抗体(OLAb)ELISA试剂盒 LDL oxidation mouse antibody (OLAb) ELISA Kit
yb-E11968 小鼠抗平滑肌抗体(ASMA)ELISA试剂盒 Mouse anti-smooth muscle antibodies (ASMA) ELISA Kit
yb-E11969 小鼠胚胎干细胞系MESPU30(M30)ELISA试剂盒 Mouse embryonic stem cell lines MESPU30 (M30) ELISA kit
yb-E11970 小鼠核因子κB亚基p65亲和肽(NF-κB p65)ELISA试剂盒 Mice nuclear factor κB p65 subunit affinity peptide (NF-κB p65) ELISA kit
yb-E11971 小鼠血小板膜糖蛋白ⅡbⅢa(GP-ⅡbⅢa/CD41+CD61)ELISA试剂盒 Mouse platelet membrane glycoprotein Ⅱ b Ⅲ a (GP-Ⅱ b Ⅲ a/CD41 + CD61) ELISA Kit
yb-E11972 小鼠游离脂肪酸(FFA)ELISA试剂盒 Mice free fatty acid (FFA) ELISA Kit
yb-E11973 小鼠血小板反应蛋白/凝血酶敏感蛋白1(TSP-1)ELISA试剂盒 Mouse platelet -reactive protein / thrombospondin 1 (TSP-1) ELISA Kit
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
人神经营养因子3 (NT -3 ) 酶联免疫分析（ELISA ） 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中 神经营养因子3 (NT -3 ) 的 含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中 人 脑源性神经营养因子（BDNF ） 水平。用纯化的 抗 - 脑源性神经营养因子（BDNF ） 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次
应用案例 | 抑郁症患者的福音，Cell 杂志发文揭示心理韧性的环路机制
初级听觉皮层中接受来自同侧内侧膝状体兴奋性神经元投射的 PV 神经元在慢性社交失败模型中的激活是心理韧性产生的充分必要条件。 CSDS 模型的早期阶段中，应激事件诱发投射到 A1 的 MG 神经元的短期超极化；在随后的应激反应中，通过突触后 glua2 型 AMPA 受体的稳态调节机制，加强 MGPV 神经元和 MGPV 神经元之间的突触传递，同时脑源性神经营养因子（brain-derived neurotrophic factor, BDNF）「供体」神经元被激活并释放 BDNF，BDNF 作用
亨廷顿舞蹈症 | 一文理清mHTT在HD发病机制中的核心枢纽作用
脑源性神经营养因子（BDNF）等关键基因表达，并引起染色质修饰异常。 细胞内环境方面，蛋白酶体功能障碍导致蛋白质稳态失衡；线粒体受损引发钙稳态失调、动力学异常及ATP生成减少，加剧氧化应激。突触层面，NMDA受体功能异常及谷氨酸摄取障碍导致兴奋性毒性，同时轴突运输受阻影响细胞器与递质递送。此外，神经胶质细胞功能紊乱，小胶质细胞释放促炎因子，星形胶质细胞摄取谷氨酸能力下降，共同加速神经元死亡。这些机制交织作用，最终导致纹状体等脑区神经元进行性丢失。 亨廷顿舞蹈病治疗 亨廷顿舞蹈症（HD）的治疗强调多学科协作，需
技术资料暂无技术资料 索取技术资料
脑源性神经营养因子(BDNF)检测试剂盒
¥900 - 1890
