- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YBEA551Hu
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- 详细信息
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Homo sapiens (Human，人)
碱性成纤维细胞生长因子(FGF2)检测试剂盒
检测范围 12.35-1000pg/mL 灵敏度 4.66pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长 2.5h 实验方法 竞争抑制法
规格 96T
碱性成纤维细胞生长因子(FGF2)检测试剂盒
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yb-E11724 小鼠粒细胞集落刺激因子(G-CSF)ELISA试剂盒 Mouse granulocyte colony -stimulating factor (G-CSF) ELISA kit
yb-E11725 小鼠细胞间粘附分子1(ICAM-1/CD54)ELISA试剂盒 Murine cell adhesion molecule 1 (ICAM-1/CD54) ELISA kit
yb-E11726 小鼠胰岛素样生长因子1(IGF-1)ELISA试剂盒 Mouse insulin-like growth factor 1 (IGF-1) ELISA Kit
yb-E11727 小鼠白介素10(IL-10)ELISA试剂盒 Murine interleukin- 10 (IL-10) ELISA kit
yb-E11728 小鼠白介素11(IL-11)ELISA试剂盒 Murine interleukin- 11 (IL-11) ELISA kit
yb-E11729 小鼠白介素15(IL-15)ELISA试剂盒 Murine interleukin- 15 (IL-15) ELISA kit
yb-E11730 小鼠17羟皮质类固醇(17-OHCS)ELISA试剂盒 17-hydroxy corticosteroids mice (17-OHCS) ELISA kit
yb-E11731 小鼠白介素1α(IL-1α)ELISA试剂盒 Murine interleukin- 1α (IL-1α) ELISA kit
yb-E11732 小鼠白介素2(IL-2)ELISA试剂盒 Murine interleukin- 2 (IL-2) ELISA Kit
yb-E11733 小鼠白介素6(IL-6)ELISA试剂盒 Murine interleukin- 6 (IL-6) ELISA Kit
yb-E11734 小鼠白介素9(IL-9)ELISA试剂盒 Murine interleukin- 9 (IL-9) ELISA kit
yb-E11735 小鼠苗条素受体(LR/Ob-R)ELISA试剂盒 Mice slim receptor (LR / Ob-R) ELISA Kit
yb-E11736 小鼠瘦素(LEP)ELISA试剂盒 Mouse leptin (LEP) ELISA Kit
yb-E11737 小鼠白血病抑制因子(LIF)ELISA试剂盒 Mouse leukemia inhibitory factor (LIF) ELISA Kit
yb-E11738 小鼠巨噬细胞炎性蛋白1β(MIP-1β/CCL4)ELISA试剂盒 Murine macrophage inflammatory protein 1β (MIP-1β/CCL4) ELISA kit
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碱性成纤维细胞生长因子(FGF2)检测试剂盒
检测范围 12.35-1000pg/mL 灵敏度 4.66pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长 2.5h 实验方法 竞争抑制法
规格 96T
碱性成纤维细胞生长因子(FGF2)检测试剂盒
ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | CEA551Hu |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Format | 96T |
| Assay length | 2.5 hours |
| Detection range | 12.35-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 333.33pg/mL, 111.11pg/mL, 37.04pg/mL, 12.35pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 4.66pg/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 2, Basic (FGF2).
No significant cross-reactivity or interference between Fibroblast Growth Factor 2, Basic (FGF2) and analogues was observed.
No significant cross-reactivity or interference between Fibroblast Growth Factor 2, Basic (FGF2) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 2, Basic (FGF2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 2, Basic (FGF2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 2, Basic (FGF2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
碱性成纤维细胞生长因子(FGF2)检测试剂盒The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1 | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| Reagent Diluent | 1×300µL | Stop Solution | 1×6mL |
| TMB Substrate | 1×9mL | Instruction manual | 1 |
| Wash Buffer (30 × concentrate) | 1×20mL |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37oC;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37oC;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle
碱性成纤维细胞生长因子(FGF2)检测试剂盒This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Fibroblast Growth Factor 2, Basic (FGF2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Fibroblast Growth Factor 2, Basic (FGF2) and unlabeled Fibroblast Growth Factor 2, Basic (FGF2) (Standards or samples) with the pre-coated antibody specific to Fibroblast Growth Factor 2, Basic (FGF2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibroblast Growth Factor 2, Basic (FGF2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Fibroblast Growth Factor 2, Basic (FGF2) in the sample.
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yb-E11725 小鼠细胞间粘附分子1(ICAM-1/CD54)ELISA试剂盒 Murine cell adhesion molecule 1 (ICAM-1/CD54) ELISA kit
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yb-E11730 小鼠17羟皮质类固醇(17-OHCS)ELISA试剂盒 17-hydroxy corticosteroids mice (17-OHCS) ELISA kit
yb-E11731 小鼠白介素1α(IL-1α)ELISA试剂盒 Murine interleukin- 1α (IL-1α) ELISA kit
yb-E11732 小鼠白介素2(IL-2)ELISA试剂盒 Murine interleukin- 2 (IL-2) ELISA Kit
yb-E11733 小鼠白介素6(IL-6)ELISA试剂盒 Murine interleukin- 6 (IL-6) ELISA Kit
yb-E11734 小鼠白介素9(IL-9)ELISA试剂盒 Murine interleukin- 9 (IL-9) ELISA kit
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碱性成纤维细胞生长因子(FGF2)检测试剂盒
¥900 - 1890
