- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YBEA114Hu
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- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Homo sapiens (Human，人)
胎盘生长因子(PLGF)检测试剂盒
检测范围 15.63-1000pg/mL 灵敏度 5.4pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
胎盘生长因子(PLGF)检测试剂盒
yb-E11463 人细胞膜表面免疫球蛋白(SmIg)ELISA试剂盒 Human cell surface immunoglobulin (SmIg) ELISA Kit
yb-E11464 人前白蛋白(PA)ELISA试剂盒 Human prealbumin (PA) ELISA Kit
yb-E11465 人硫酸肝素糖蛋白(HSPG)ELISA试剂盒 Human glycoprotein heparan sulfate (HSPG) ELISA Kit
yb-E11466 人冷球蛋白(CG)ELISA试剂盒 People cryoglobulins (CG) ELISA kit
yb-E11467 人红细胞膜蛋白(EMP)ELISA试剂盒 Human erythrocyte membrane protein (EMP) ELISA kit
yb-E11468 人高铁血红蛋白(MHB)ELISA试剂盒 Human methemoglobin (MHB) ELISA kit
yb-E11469 人低分子肝素(LMWH)ELISA试剂盒 People LMWH (LMWH) ELISA kit
yb-E11470 人补体3裂解产物(C3SP)ELISA试剂盒 Cleavage products of human complement 3 (C3SP) ELISA Kit
yb-E11471 人补体片段3b受体(C3bR)ELISA试剂盒 3b human complement receptor fragments (C3bR) ELISA Kit
yb-E11472 人补体1抑制物抗体(C1INH)ELISA试剂盒 An antibody of human complement inhibitor (C1INH) ELISA Kit
yb-E11473 人B因子(BF)ELISA试剂盒 Human factor B (BF) ELISA Kit
yb-E11474 人类白细胞抗原G(HLA-G)ELISA试剂盒 Human leukocyte antigen G (HLA-G) ELISA kit
yb-E11475 人补体片断3b(C3b)ELISA试剂盒 Human complement fragment 3b (C3b) ELISA Kit
yb-E11476 人补体片断4b(C4b)ELISA试剂盒 Human complement fragment 4b (C4b) ELISA Kit
yb-E11477 人补体片断5b(C5b)ELISA试剂盒 Human complement fragment 5b (C5b) ELISA Kit
yb-E11478 人免疫球蛋白重链(IgH)ELISA试剂盒 Human immunoglobulin heavy chain (IgH) ELISA Kit
yb-E11479 人免疫球蛋白重链可变区(IgHV)ELISA试剂盒 Human immunoglobulin heavy chain variable region (IgHV) ELISA Kit
yb-E11480 人多免疫球蛋白受体(poly-IgR)ELISA试剂盒 People immunoglobulin receptor (poly-IgR) ELISA Kit
yb-E11481 人血红蛋白晚期糖基化终末产物(Hb-AGE)ELISA试剂盒 Human hemoglobin advanced glycation end products (Hb-AGE) ELISA kit
yb-E11482 人表面膜免疫球蛋白A(mIgA)ELISA试剂盒 Mask human immunoglobulin Table A (mIgA) ELISA kit
yb-E11483 人免疫球蛋白j链(Ig-j)ELISA试剂盒 J chain of human immunoglobulin (Ig-j) ELISA Kit
yb-E11484 人血红蛋白β亚基(HB-β)ELISA试剂盒 Β subunit of human hemoglobin (HB-β) ELISA Kit
yb-E11485 人分泌型免疫球蛋白A(SIgA)ELISA试剂盒 Human secretory immunoglobulin A (SIgA) ELISA kit
yb-E11486 人活化凝血因子Ⅻ(FⅫa)ELISA试剂盒 Human activated coagulation factor Ⅻ (F Ⅻ a) ELISA kit
胎盘生长因子(PLGF)检测试剂盒
检测范围 15.63-1000pg/mL 灵敏度 5.4pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
胎盘生长因子(PLGF)检测试剂盒
ELISA Kit for Placenta Growth Factor (PLGF)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | SEA114Hu |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 15.63-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 5.4pg/mL. |
Specificity
胎盘生长因子(PLGF)检测试剂盒This assay has high sensitivity and excellent specificity for detection of Placenta Growth Factor (PLGF).
No significant cross-reactivity or interference between Placenta Growth Factor (PLGF) and analogues was observed.
No significant cross-reactivity or interference between Placenta Growth Factor (PLGF) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Placenta Growth Factor (PLGF) and the recovery rates were calculated by comparing the measured value to the expected amount of Placenta Growth Factor (PLGF) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 95-103 | 98 |
| EDTA plasma(n=5) | 94-103 | 99 |
| heparin plasma(n=5) | 83-97 | 88 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Placenta Growth Factor (PLGF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Placenta Growth Factor (PLGF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Placenta Growth Factor (PLGF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Placenta Growth Factor (PLGF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-103% | 81-96% | 97-105% | 83-91% |
| EDTA plasma(n=5) | 95-104% | 88-96% | 83-94% | 79-92% |
| heparin plasma(n=5) | 85-103% | 90-98% | 82-98% | 91-99% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
胎盘生长因子(PLGF)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Placenta Growth Factor (PLGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Placenta Growth Factor (PLGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Placenta Growth Factor (PLGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Placenta Growth Factor (PLGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
yb-E11463 人细胞膜表面免疫球蛋白(SmIg)ELISA试剂盒 Human cell surface immunoglobulin (SmIg) ELISA Kit
yb-E11464 人前白蛋白(PA)ELISA试剂盒 Human prealbumin (PA) ELISA Kit
yb-E11465 人硫酸肝素糖蛋白(HSPG)ELISA试剂盒 Human glycoprotein heparan sulfate (HSPG) ELISA Kit
yb-E11466 人冷球蛋白(CG)ELISA试剂盒 People cryoglobulins (CG) ELISA kit
yb-E11467 人红细胞膜蛋白(EMP)ELISA试剂盒 Human erythrocyte membrane protein (EMP) ELISA kit
yb-E11468 人高铁血红蛋白(MHB)ELISA试剂盒 Human methemoglobin (MHB) ELISA kit
yb-E11469 人低分子肝素(LMWH)ELISA试剂盒 People LMWH (LMWH) ELISA kit
yb-E11470 人补体3裂解产物(C3SP)ELISA试剂盒 Cleavage products of human complement 3 (C3SP) ELISA Kit
yb-E11471 人补体片段3b受体(C3bR)ELISA试剂盒 3b human complement receptor fragments (C3bR) ELISA Kit
yb-E11472 人补体1抑制物抗体(C1INH)ELISA试剂盒 An antibody of human complement inhibitor (C1INH) ELISA Kit
yb-E11473 人B因子(BF)ELISA试剂盒 Human factor B (BF) ELISA Kit
yb-E11474 人类白细胞抗原G(HLA-G)ELISA试剂盒 Human leukocyte antigen G (HLA-G) ELISA kit
yb-E11475 人补体片断3b(C3b)ELISA试剂盒 Human complement fragment 3b (C3b) ELISA Kit
yb-E11476 人补体片断4b(C4b)ELISA试剂盒 Human complement fragment 4b (C4b) ELISA Kit
yb-E11477 人补体片断5b(C5b)ELISA试剂盒 Human complement fragment 5b (C5b) ELISA Kit
yb-E11478 人免疫球蛋白重链(IgH)ELISA试剂盒 Human immunoglobulin heavy chain (IgH) ELISA Kit
yb-E11479 人免疫球蛋白重链可变区(IgHV)ELISA试剂盒 Human immunoglobulin heavy chain variable region (IgHV) ELISA Kit
yb-E11480 人多免疫球蛋白受体(poly-IgR)ELISA试剂盒 People immunoglobulin receptor (poly-IgR) ELISA Kit
yb-E11481 人血红蛋白晚期糖基化终末产物(Hb-AGE)ELISA试剂盒 Human hemoglobin advanced glycation end products (Hb-AGE) ELISA kit
yb-E11482 人表面膜免疫球蛋白A(mIgA)ELISA试剂盒 Mask human immunoglobulin Table A (mIgA) ELISA kit
yb-E11483 人免疫球蛋白j链(Ig-j)ELISA试剂盒 J chain of human immunoglobulin (Ig-j) ELISA Kit
yb-E11484 人血红蛋白β亚基(HB-β)ELISA试剂盒 Β subunit of human hemoglobin (HB-β) ELISA Kit
yb-E11485 人分泌型免疫球蛋白A(SIgA)ELISA试剂盒 Human secretory immunoglobulin A (SIgA) ELISA kit
yb-E11486 人活化凝血因子Ⅻ(FⅫa)ELISA试剂盒 Human activated coagulation factor Ⅻ (F Ⅻ a) ELISA kit
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胎盘生长因子(PLGF)检测试剂盒
¥900 - 1890
