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- 钰博生物
- 见说明书
- 德国/美国/中国
- YBEA041Hu
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Homo sapiens (Human，人)
中性粒细胞激活蛋白3(NAP3)检测试剂盒
检测范围 15.63-1000pg/mL 灵敏度 5.7pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
中性粒细胞激活蛋白3(NAP3)检测试剂盒
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中性粒细胞激活蛋白3(NAP3)检测试剂盒
检测范围 15.63-1000pg/mL 灵敏度 5.7pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
中性粒细胞激活蛋白3(NAP3)检测试剂盒
ELISA Kit for Neutrophil Activating Protein 3 (NAP3)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | SEA041Hu |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 15.63-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 5.7pg/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Neutrophil Activating Protein 3 (NAP3).
No significant cross-reactivity or interference between Neutrophil Activating Protein 3 (NAP3) and analogues was observed.
No significant cross-reactivity or interference between Neutrophil Activating Protein 3 (NAP3) and analogues was observed.
Recovery
中性粒细胞激活蛋白3(NAP3)检测试剂盒Matrices listed below were spiked with certain level of recombinant Neutrophil Activating Protein 3 (NAP3) and the recovery rates were calculated by comparing the measured value to the expected amount of Neutrophil Activating Protein 3 (NAP3) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 97-105 | 101 |
| EDTA plasma(n=5) | 83-99 | 90 |
| heparin plasma(n=5) | 92-101 | 95 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neutrophil Activating Protein 3 (NAP3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neutrophil Activating Protein 3 (NAP3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neutrophil Activating Protein 3 (NAP3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neutrophil Activating Protein 3 (NAP3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-101% | 90-98% | 80-93% | 80-99% |
| EDTA plasma(n=5) | 82-98% | 85-96% | 89-96% | 90-104% |
| heparin plasma(n=5) | 89-105% | 80-102% | 97-105% | 80-92% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
中性粒细胞激活蛋白3(NAP3)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutrophil Activating Protein 3 (NAP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neutrophil Activating Protein 3 (NAP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neutrophil Activating Protein 3 (NAP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neutrophil Activating Protein 3 (NAP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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中性粒细胞激活蛋白3(NAP3)检测试剂盒
¥900 - 1890
