- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YBEA050Hu
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Homo sapiens (Human，人)
胰岛素样生长因子1(IGF1)检测试剂盒
检测范围 0.188-12ng/mL 灵敏度 0.09ng/mL
样本类型 Serum, plasma and other biological fluids
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
胰岛素样生长因子1(IGF1)检测试剂盒
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yb-E10373 人乙酰肝素酶(HPA)ELISA试剂盒 Human heparanase (HPA) ELISA kit
yb-E10374 人Ⅱ型胶原螺旋肽(HELIX-Ⅱ)ELISA试剂盒 People Ⅱ collagen helical peptide (HELIX-Ⅱ) ELISA kit
yb-E10375 人纤维连接素相关抗原(FRA)ELISA试剂盒 Human fibronectin associated antigen (FRA) ELISA kit
yb-E10376 人胃泌素细胞抗体(GCA)ELISA试剂盒 Human gastrin cell antibodies (GCA) ELISA Kit
yb-E10377 人谷氨酸脱氢酶(GDH/GLDH)ELISA试剂盒 Human glutamate dehydrogenase (GDH / GLDH) ELISA kit
yb-E10378 人肝素辅因子Ⅱ(HCⅡ)ELISA试剂盒 People heparin cofactor Ⅱ (HC Ⅱ) ELISA kit
yb-E10379 人胆酸(CA)ELISA试剂盒 Human acid (CA) ELISA Kit
yb-E10380 人幽门螺旋菌IgG(Hp-IgG)ELISA试剂盒 People Helicobacter pylori IgG (Hp-IgG) ELISA Kit
yb-E10381 人γ谷氨酰转移酶(GGT) ELISA试剂盒 Human γ -glutamyl transferase (GGT) ELISA kit
yb-E10382 人胃粘液素(GM)ELISA试剂盒 Human gastric mucin (GM) ELISA kit
yb-E10383 人透明质酸结合蛋白(HABP)ELISA试剂盒 Human hyaluronan binding protein (HABP) ELISA Kit
yb-E10384 人Ⅰ型胶原N末端肽(NTX)ELISA试剂盒 Human N -terminal peptide of collagen type Ⅰ (NTX) ELISA Kit
yb-E10385 人幽门螺旋杆菌IgM(Hp-IgM)ELISA试剂盒 Helicobacter pylori human IgM (Hp-IgM) ELISA Kit
yb-E10386 人细胞毒素相关蛋白A(CagA)ELISA试剂盒 People cytotoxin -associated protein A (CagA) ELISA kit
yb-E10387 人胃抑素(GIP)ELISA试剂盒 Human GIP (GIP) ELISA kit
yb-E10388 人胃泌素释放多肽(GRP)ELISA试剂盒 Human gastrin-releasing peptide (GRP) ELISA kit
yb-E10389 人胃泌素释放肽前体(ProGRP)ELISA试剂盒 Human gastrin-releasing peptide precursor (ProGRP) ELISA kit
yb-E10390 人胶原蛋白Ⅱ(HCBⅡ)ELISA试剂盒 Collagen Ⅱ (HCB Ⅱ) ELISA kit
yb-E10391 人促胰液素/胰泌素(Secretin)ELISA试剂盒 Human secretin / secretin (Secretin) ELISA kit
yb-E10392 人多肽YY(Peptide-YY)ELISA试剂盒 Human peptide YY (Peptide-YY) ELISA kit
胰岛素样生长因子1(IGF1)检测试剂盒
检测范围 0.188-12ng/mL 灵敏度 0.09ng/mL
样本类型 Serum, plasma and other biological fluids
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
胰岛素样生长因子1(IGF1)检测试剂盒
ELISA Kit for Insulin Like Growth Factor 1 (IGF1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | SEA050Hu |
| Sample type | Serum, plasma and other biological fluids |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 0.188-12ng/mL The standard curve concentrations used for the ELISA’s were 12ng/mL, 6ng/mL, 3ng/mL, 1.5ng/mL, 0.75ng/mL, 0.375ng/mL, 0.188ng/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 0.09ng/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor 1 (IGF1).
No significant cross-reactivity or interference between Insulin Like Growth Factor 1 (IGF1) and analogues was observed.
No significant cross-reactivity or interference between Insulin Like Growth Factor 1 (IGF1) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Insulin Like Growth Factor 1 (IGF1) and the recovery rates were calculated by comparing the measured value to the expected amount of Insulin Like Growth Factor 1 (IGF1) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 92-102 | 98 |
| EDTA plasma(n=5) | 81-92 | 86 |
| heparin plasma(n=5) | 95-104 | 101 |
Precision
胰岛素样生长因子1(IGF1)检测试剂盒Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor 1 (IGF1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor 1 (IGF1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor 1 (IGF1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Insulin Like Growth Factor 1 (IGF1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-105% | 84-95% | 91-103% | 84-103% |
| EDTA plasma(n=5) | 90-103% | 84-99% | 82-104% | 81-89% |
| heparin plasma(n=5) | 81-88% | 86-95% | 79-101% | 81-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
胰岛素样生长因子1(IGF1)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 1 (IGF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 1 (IGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor 1 (IGF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor 1 (IGF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
yb-E10367 人Ⅰ型胶原交联羧基末端肽(ⅠCTP)ELISA试剂盒 People Ⅰ collagen crosslinking carboxyl terminal peptide (Ⅰ CTP) ELISA kit
yb-E10368 人Ⅰ型胶原C端肽(CTX-Ⅰ)ELISA试剂盒 Human collagen type Ⅰ C -terminal peptide (CTX-Ⅰ) ELISA kit
yb-E10369 人粘蛋白/粘液素5AC(MUC5AC)ELISA试剂盒 Human mucin / mucin 5AC (MUC5AC) ELISA Kit
yb-E10370 人丙氨酸转氨酶/谷丙转氨酶(ALT/GPT)ELISA试剂盒 People alanine aminotransferase / alanine aminotransferase (ALT / GPT) ELISA kit
yb-E10371 人天门冬氨酸转氨酶/谷草转氨酶(GOT/GPT)ELISA试剂盒 People aspartate aminotransferase / aspartate transaminase (GOT / GPT) ELISA kit
yb-E10372 人肝脂酶(HL)ELISA试剂盒 Human hepatic lipase (HL) ELISA kit
yb-E10373 人乙酰肝素酶(HPA)ELISA试剂盒 Human heparanase (HPA) ELISA kit
yb-E10374 人Ⅱ型胶原螺旋肽(HELIX-Ⅱ)ELISA试剂盒 People Ⅱ collagen helical peptide (HELIX-Ⅱ) ELISA kit
yb-E10375 人纤维连接素相关抗原(FRA)ELISA试剂盒 Human fibronectin associated antigen (FRA) ELISA kit
yb-E10376 人胃泌素细胞抗体(GCA)ELISA试剂盒 Human gastrin cell antibodies (GCA) ELISA Kit
yb-E10377 人谷氨酸脱氢酶(GDH/GLDH)ELISA试剂盒 Human glutamate dehydrogenase (GDH / GLDH) ELISA kit
yb-E10378 人肝素辅因子Ⅱ(HCⅡ)ELISA试剂盒 People heparin cofactor Ⅱ (HC Ⅱ) ELISA kit
yb-E10379 人胆酸(CA)ELISA试剂盒 Human acid (CA) ELISA Kit
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yb-E10381 人γ谷氨酰转移酶(GGT) ELISA试剂盒 Human γ -glutamyl transferase (GGT) ELISA kit
yb-E10382 人胃粘液素(GM)ELISA试剂盒 Human gastric mucin (GM) ELISA kit
yb-E10383 人透明质酸结合蛋白(HABP)ELISA试剂盒 Human hyaluronan binding protein (HABP) ELISA Kit
yb-E10384 人Ⅰ型胶原N末端肽(NTX)ELISA试剂盒 Human N -terminal peptide of collagen type Ⅰ (NTX) ELISA Kit
yb-E10385 人幽门螺旋杆菌IgM(Hp-IgM)ELISA试剂盒 Helicobacter pylori human IgM (Hp-IgM) ELISA Kit
yb-E10386 人细胞毒素相关蛋白A(CagA)ELISA试剂盒 People cytotoxin -associated protein A (CagA) ELISA kit
yb-E10387 人胃抑素(GIP)ELISA试剂盒 Human GIP (GIP) ELISA kit
yb-E10388 人胃泌素释放多肽(GRP)ELISA试剂盒 Human gastrin-releasing peptide (GRP) ELISA kit
yb-E10389 人胃泌素释放肽前体(ProGRP)ELISA试剂盒 Human gastrin-releasing peptide precursor (ProGRP) ELISA kit
yb-E10390 人胶原蛋白Ⅱ(HCBⅡ)ELISA试剂盒 Collagen Ⅱ (HCB Ⅱ) ELISA kit
yb-E10391 人促胰液素/胰泌素(Secretin)ELISA试剂盒 Human secretin / secretin (Secretin) ELISA kit
yb-E10392 人多肽YY(Peptide-YY)ELISA试剂盒 Human peptide YY (Peptide-YY) ELISA kit
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文献和实验相关实验
的物理性嗅觉干预即可显著抑制同侧嗅球中肿瘤的发生与发展。综上，利用化学遗传学以及物理方法，研究团队充分证明了嗅觉感知可以调控胶质瘤的发生。 利用硅胶塞剥夺小鼠嗅觉可以抑制嗅球中胶质瘤 接下来，研究团队对嗅觉调控胶质瘤发生的分子机制进行了探究。 通过对单侧嗅觉剥夺小鼠的两侧嗅球组织进行转录组测序，筛选出了潜在效应因子- 胰岛素样生长因子 1（IGF1）。 有趣的是，团队之前发表的工作已经证明了前癌细胞表达的 IGF1 受体（IGF1R）对胶质瘤发生发展具有重要作用，支持对 IGF1
症综合征的遗传热点。由于WAPCre系统，这种点突变只在小鼠的乳腺中活跃，通过这种突变，该菌株将在1岁左右自发地发展出与人类相关的乳腺肿瘤。我们经常让这些小鼠模型接触胰岛素样分子。频繁注射两种模型化合物AspB10和胰岛素样生长因子-1（IGF1）后，大鼠乳腺肿瘤的表达明显降低。AspB10，又称X10，是一种早期胰岛素类似物，由于增强了IGF1R结合亲和力，具有很强的启动细胞分裂的潜力。IGF1是IGF1R的天然配体，这种受体在细胞分裂信号中起着重要作用。与常规胰岛素相比，甘精胰岛素在体外实验中
的点突变而得名;一种与人类李弗罗梅尼癌症综合征基因热点相对应的基因。由于WAPCre系统，该位点突变只在小鼠乳腺中活跃，在1岁左右，该菌株会自发地发展为人类相关乳腺肿瘤。我们经常将这些小鼠模型暴露在类胰岛素分子中。频繁注射两种模型化合物AspB10和胰岛素样生长因子-1 (IGF1)后，肿瘤表达明显下降，这两种模型化合物均与大鼠乳腺肿瘤相关。AspB10，也被称为X10，是一种早期的胰岛素类似物，由于增强了IGF1R的结合亲和力，具有启动细胞分裂的强大潜力。IGF1是IGF1R的天然配体，该受体在细胞
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胰岛素样生长因子1(IGF1)检测试剂盒
¥900 - 1890
