Ras Activation Assay Biochem K

Product Uses Include

Ras Activation Assay Biochem KitIntroduction
The Ras switch operates by alternating between an active, GTP-bound state and an inactive, GDP-bound state. Understanding the mechanisms that regulate activation / inactivation of Ras-like GTPases is of obvious biological significance and is a subject of intense investigation. The fact that Ras family effector proteins will specifically recognize the GTP bound form of the protein has been exploited experimentally to develop a powerful affinity purification assay that monitors Ras protein activation. The assay uses the Ras-binding domain (RBD) of the Ras effector kinase Raf1. The Raf-RBD domain has been shown to bind specifically to the GTP-bound form of Ras proteins. The fact that the Raf-RBD has a high affinity for GTP-Ras and that its binding results in a significantly reduced intrinsic and catalytic rate of hydrolysis of Ras make it an ideal tool for affinity purification of GTP-Ras from cell lysates. The Raf-RBD is in the form of a GST fusion protein, which allows one to "pull-down" the Raf-RBD/GTP-Ras complex with glutathione affinity beads. The assay therefore provides a simple means of quantitating Ras activation in cells. The amount of activated Ras is determined by a western blot using a Ras specific antibody.
Ras Activation Assay Biochem Kit
xy-0236xy ChRM2 (Muscarinic Acetylcholine receptor M2)毒蕈碱型乙酰胆碱受体（抗原）
xy-0274xy C-Peptide( C-PEP )C-肽（C肽）
xy-0062xy CR (Calretinin)钙结合蛋白（抗原）
xy-1225xy ADORA3/A3AR(adenosine receptor A3)腺苷受体A3抗原
xy-2203xy Integrin alpha V巨噬细胞来源的趋化因子αV多肽
xy-5551xy phospho-AMPK alpha-1/PRKAA1(Thr198) peptide磷酸化腺苷单磷酸活化蛋白激酶α1抗原
xy-0035xy CREB-1 (cAMP responsive element binding protein-1)环腺苷酸应答元件结合蛋白（抗原）
xy-0036xy phospho-CREB-1 磷酸化环腺苷酸应答元件结合蛋白（多肽）
xy-1062xy ADRA2 peptide肾上腺素能受体2抗原
xy-0246xy CRF (Corticotropin-Releasing Factor)促肾上腺皮质激素释放因子
xy-0063xy CT (Calcitonin )降钙素（抗原）
xy-0124xy CT-R (Calcitonin receptor)降钙素受体（抗原）
xy-5907xy Ferritin peptide铁蛋白抗原
xy-0013xy Cytochrome C细胞色素 C（抗原）
xy-1063xy ADRB3/ Beta3-AR ( Beta3-adrenergic receptor)肾上腺素能受体β3
xy-0271xy Cytomegalovirus (CMV)PP65细胞巨化病毒(多肽)
xy-0180xy DDC (DOPA decarboxylase)多巴胺脱羧酶（抗原）
xy-0405xy EGFR- V III人表皮生长因子受体-8（多肽片断抗原）
xy-2045xy Alpha-AF/ Alpha-Afa(alpha-L-arabinofuranosidase)α-L-阿拉伯呋喃糖苷酶抗原
xy-6282xy SCCA peptide鳞状细胞癌抗原
xy-0202xy Endothelin-1内皮素-1(多肽抗原)
xy-0188xy Endothelin-1 (ET-1)内皮素-1（抗原）
xy-0021xy ERAB 内质网Aβ相关结合蛋白（抗原）
xy-6618xy PAPPA妊娠相关血浆蛋白A
xy-0415xy TIMP-1(Tissue Inhibitor of Metalloproteinase-1)金属蛋白酶组织抑制因子-1（抗原）
xy-1223xy Aggrecan软骨蛋白聚糖/可聚蛋白多糖抗原
xy-0243xy EXPB-2植物延展素-β（抗原）
xy-0215xy FAS (Apo-a1; CD95;)(抗原)载脂蛋白-a1
xy-2132xy Agtr1/AT1a(Angiotensin II receptor, type 1a)血管组织血管紧张素Ⅱ1型受体抗原
xy-0216xy FasL(抗原)
xy-1416xy Ah Receptor(Aryl Hydrocarbon Receptor)芳香烃受体抗原
xy-0110xy Fe65 protein peptideFe65 蛋白（多肽抗原）
xy-0229xy FGF1 (aFGF )(ECGF-beta)(HBGF-1)肝素结合生长因子( 酸性成纤维细胞生长因子）（多肽片断抗原）
xy-0315xy fgL2 (Fibrinogen-like 2)凝血酶原酶 (又称：纤维介素蛋白；前凝血素)
Ras Activation Assay Biochem Kitxy-0119xy FLIP S/L peptide凋亡调节基因之一（多肽抗原）
xy-0269xy FoxP3叉头蛋白3-T细胞转录因子（多肽抗原）