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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4℃保存
- 保质期：
6个月
- 英文名：
Tubulin glycerol buffer
- 库存：
1
- 供应商：
上海信裕
- Tubulin glycerol buffer详细信息：
- Product Uses Include
- Promote tubulin polymerization
- Use as a microtubule cushion buffer
Material
Formerly called Microtubule Cushion Buffer. Contains BST01 in 60% [v/v] glycerol. When diluted in G-PEM to a final concentration of e.g. 5% glycerol, it can be used to promote tubulin polymerization. It is also used to separate microtubules from non-polymerized tubulin by centrifugation and to separate microtubule bound MAPs from non-microtubule-binding proteins.
Tubulin glycerol buffer
xy-0308R-xyy3 Rabbit Anti-horse IgG/Cy3Cy3标记的兔抗马IgG
xy-0308R-xyy5 Rabbit Anti-horse IgG/Cy5Cy5标记的兔抗马IgG
xy-0308R-xyy5.5 Rabbit Anti-horse IgG/Cy5.5Cy5.5标记的兔抗马IgG
xy-0308R-xyy7 Rabbit Anti-horse IgG/Cy7Cy7标记的兔抗马IgG
xy-0308R-xyE Rabbit Anti-horse IgG/PEPE标记的兔抗马IgG
xy-0297R-RBITxy Rabbit Anti-human IgG/RBITC罗丹明标记的兔抗人IgG
xy-0297R-Golxy Rabbit Anti-human IgG/Gold胶体金标记的兔抗人IgG
xy-0297R-Axy Rabbit Anti-human IgG/AP碱性磷酸酶（AP）标记的兔抗人IgG
xy-0297R-xyE-xyy5.5 Rabbit Anti-human IgG/PE-Cy5.5PE-Cy5.5标记的兔抗人IgG
xy-0297R-xyE-xyy7 Rabbit Anti-human IgG/PE-Cy7PE-Cy7标记的兔抗人IgG
xy-0297R-Axyxy Rabbit Anti-human IgG/APCAPC标记的兔抗人IgG
xy-0297R-AF350 Rabbit Anti-human IgG/Alexa Fluor 350Alexa Fluor 350标记的兔抗人IgG
Tubulin glycerol bufferxy-0297R-AF488 Rabbit Anti-human IgG/Alexa Fluor 488Alexa Fluor 488标记的兔抗人IgG
xy-0297R-AF555 Rabbit Anti-human IgG/Alexa Fluor 555Alexa Fluor 555标记的兔抗人IgG
xy-0297R-xyy3 Rabbit Anti-human IgG/Cy3Cy3标记的兔抗人IgG
xy-0297R-xyy5 Rabbit Anti-human IgG/Cy5Cy5标记的兔抗人IgG
xy-0297R-xyE Rabbit Anti-human IgG/PEPE标记的兔抗人IgG
xy-0297R-xyE-xyy3 Rabbit Anti-human IgG/PE-Cy3PE-Cy3标记的兔抗人IgG
xy-0297R-xyy5.5 Rabbit Anti-human IgG/Cy5.5Cy5.5标记的兔抗人IgG
xy-0297R-xyy7 Rabbit Anti-human IgG/Cy7Cy7标记的兔抗人IgG
xy-0345R-RBITxy Rabbit Anti-human IgM/RBITC罗丹明标记的兔抗人IgM
xy-0345R-Bio Rabbit Anti-human IgM/Bio生物素标记的兔抗人IgM
xy-0345R-HRxy Rabbit Anti-human IgM/HRP辣根过氧化物酶标记的兔抗人IgM
xy-0345R-Axy Rabbit Anti-human IgM/AP碱性磷酸酶（AP）标记的兔抗人IgM
xy-0345R-Golxy Rabbit Anti-human IgM/Gold胶体金标记的兔抗人IgM
xy-0345R-FITxy Rabbit Anti-human IgM/FITCFITC标记的兔抗人IgM
xy-0345R-xyy3 Rabbit Anti-human IgM/Cy3Cy3标记的兔抗人IgM
xy-0345R-xyy5 Rabbit Anti-human IgM/Cy5Cy5标记的兔抗人IgM
xy-0345R-xyy5.5 Rabbit Anti-human IgM/Cy5.5Cy5.5标记的兔抗人IgM
xy-0345R-xyy7 Rabbit Anti-human IgM/Cy7Cy7标记的兔抗人IgM
xy-0345R-xyE Rabbit Anti-human IgM/PEPE标记的兔抗人IgM
xy-0345R-xyE-xyy3 Rabbit Anti-human IgM/PE-Cy3PE-Cy3标记的兔抗人IgM
xy-0345R-xyE-xyY5 Rabbit Anti-human IgM/PE-CY5PE-CY5标记的兔抗人IgM
Tubulin glycerol bufferxy-0345R-Axyxy Rabbit Anti-human IgM/APCAPC标记的兔抗人IgM
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文献和实验SDS Gel Electrophoresis of Tubulin\MAPs
Buffer 0.025 M Tris 0.192 M Glycine 0.1% SDS Adjust pH to 8.3 0.2-0.3 ml samples of brain extract, and containing 200 micrograms protein 0.0625 M Tris-HCl (pH 6.8)
Thaw tubulin Adjust solution to a final buffer concentration equivalent of glycerol PB Incubate at 37 o C for 30 min. Layer tubulin onto cushions of BRB80 containing 60% (v/v) glycerol
Magnesium Sulfate 2 mM DTT PM-4M Buffer = 100 mM Pipes, pH 6.9 2 mM EGTA 1 mM Magnesium Sulfate 2 mM DTT 4 M Glycerol PM-8M Buffer = 100 mM
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