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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4℃保存
- 保质期:
6个月
- 英文名:
MCF-7 cell tubulin, biotinylated
- 库存:
1
- 供应商:
上海信裕
- MCF-7 cell tubulin, biotinylated详细信息:
- Product uses include
- Screening for cancer tubulin specific ligands.
- Using SPA technology (Amersham) for drug screening.
- Microinjection and tubulin distribution in living cells.
- Immobilizing tubulin onto a solid surface via streptavidin.
Material
MCF-7 cell tubulin (Cat. # H005) has been modified so that random surface lysines contain a covalently linked, long-chain biotin derivative. A long-chain biotin derivative was selected for this procedure because it allows the biotin molecules to be spaced far enough away from the tubulin protein so as not to interfere with its activity, e.g., ligand binding to SPA and other streptavidin based reagents. Biotin labeled tubulin is supplied as a lyophilized powder.
MCF-7 cell tubulin, biotinylated
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xy-0293G-FITxy Goat Anti-rat IgG/FITCFITC标记的羊抗大鼠IgG
xy-0293G-HRxy Goat Anti-rat IgG/HRP辣根过氧化物酶标记的羊抗大鼠IgG
xy-0293G-Bio Goat Anti-rat IgG/Bio生物素标记的羊抗大鼠IgG
xy-0293G-xyE-xyy3 Goat Anti-rat IgG/PE-Cy3PE-Cy3标记的羊抗大鼠IgG
xy-0293G-xyE-xyY5 Goat Anti-rat IgG/PE-CY5PE-CY5标记的羊抗大鼠IgG
xy-0293G-Axyxy Goat Anti-rat IgG/APCAPC标记的羊抗大鼠IgG
xy-0293G-AF350 Goat Anti-rat IgG/Alexa Fluor 350Alexa Fluor 350标记的羊抗大鼠IgG
xy-0293G-AF488 Goat Anti-rat IgG/Alexa Fluor 488Alexa Fluor 488标记的羊抗大鼠IgG
xy-0293G-xyE-xyy5.5 Goat Anti-rat IgG/PE-Cy5.5PE-Cy5.5标记的羊抗大鼠IgG
xy-0293G-xyy3 Goat Anti-rat IgG/Cy3Cy3标记的羊抗大鼠IgG
xy-0293G-xyy5 Goat Anti-rat IgG/Cy5Cy5标记的羊抗大鼠IgG
xy-0293G-xyy5.5 Goat Anti-rat IgG/Cy5.5Cy5.5标记的羊抗大鼠IgG
xy-0293G-xyy7 Goat Anti-rat IgG/Cy7Cy7标记的羊抗大鼠IgG
xy-0293G-AF555 Goat Anti-rat IgG/Alexa Fluor 555Alexa Fluor 555标记的羊抗大鼠IgG
xy-0293G-AF647 Goat Anti-rat IgG/Alexa Fluor 647Alexa Fluor 647标记的羊抗大鼠IgG
xy-0358G-HRxy Goat Anti-Guinea pig IgG/HRP辣根过氧化物酶标记的羊抗豚鼠IgG
MCF-7 cell tubulin, biotinylatedxy-0358G-Bio Goat Anti-Guinea pig IgG/Bio生物素标记的羊抗豚鼠IgG
xy-0358G-xyy5 Goat Anti-Guinea pig IgG/Cy5Cy5标记的羊抗豚鼠IgG
xy-0358G-xyy5.5 Goat Anti-Guinea pig IgG/Cy5.5Cy5.5标记的羊抗豚鼠IgG
xy-0358G-xyy7 Goat Anti-Guinea pig IgG/Cy7Cy7标记的羊抗豚鼠IgG
xy-0358G-xyE Goat Anti-Guinea pig IgG/PEPE标记的羊抗豚鼠IgG
xy-0358G-xyE-xyy3 Goat Anti-Guinea pig IgG/PE-Cy3PE-Cy3标记的羊抗豚鼠IgG
xy-0358G-xyE-xyY5 Goat Anti-Guinea pig IgG/PE-CY5PE-CY5标记的羊抗豚鼠IgG
xy-0358G-Axyxy Goat Anti-Guinea pig IgG/APCAPC标记的羊抗豚鼠IgG
xy-0358G-AF350 Goat Anti-Guinea pig IgG/Alexa Fluor 350Alexa Fluor 350标记的羊抗豚鼠IgG
xy-0358G-AF488 Goat Anti-Guinea pig IgG/Alexa Fluor 488Alexa Fluor 488标记的羊抗豚鼠IgG
xy-0358G-AF555 Goat Anti-Guinea pig IgG/Alexa Fluor 555Alexa Fluor 555标记的羊抗豚鼠IgG
xy-0358G-AF647 Goat Anti-Guinea pig IgG/Alexa Fluor 647Alexa Fluor 647标记的羊抗豚鼠IgG
xy-0358G-xyE-xyy5.5 Goat Anti-Guinea pig IgG/PE-Cy5.5PE-Cy5.5标记的羊抗豚鼠IgG
xy-0358G-xyE-xyy7 Goat Anti-Guinea pig IgG/PE-Cy7PE-Cy7标记的羊抗豚鼠IgG
xy-0358G-RBITxy Goat Anti-Guinea pig IgG/RBITC罗丹明标记的羊抗豚鼠IgG
xy-0358G-FITxy Goat Anti-Guinea pig IgG/FITCFITC标记的羊抗豚鼠IgG
xy-0358G-Golxy Goat Anti-Guinea pig IgG/Gold胶体金标记的羊抗豚鼠IgG
xy-0358G-Axy Goat Anti-Guinea pig IgG/AP碱性磷酸酶(AP)标记的羊抗豚鼠IgG
xy-0295G-Axy Goat Anti-rabbit IgG/AP碱性磷酸酶(AP)标记的羊抗兔IgG
xy-0295G-FITxy Goat Anti-rabbit IgG/FITCFITC标记的羊抗兔IgG
MCF-7 cell tubulin, biotinylatedxy-0295G-xyy7 Goat Anti-rabbit IgG/Cy7Cy7标记的羊抗兔IgG
xy-0295G-xyE-xyY5 Goat Anti-rabbit IgG/PE-CY5PE-CY5标记的羊抗兔IgG
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文献和实验Although many protocols for tubulin preparation are available, the procedure described below is the simplest and highest yielding
Western Blotting with Biotinylated Antibodies
SAMPLE PREPARATION For Protein Concentration Determination of Cell Culture 1.Decant medium from 10cm dish of adherent cells and rinse plate rapidly with phosphate-buffered saline (PBS). 2.Aspirate excess PBS. 3.Add 1ml boiling lysis buffer
Immunofluorescent Localization of Tubulin
LEVEL II Materials Coverslip cultures of an appropriate monolayer cell line Phosphate buffered saline (PBS) Acetone/Methanol (absolute) in a 50:50 volume mixture Rabbit anti-tubulin (or other primary
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