Sphingomyelinase Assay Kit

Description: The Sphingomyelinase Assay Kit provides a sensitive and homogenous method to measure the activity of sphingomyelinase in vitro.

Sphingomyelinase Assay KitFeatures

• —Homogenous enzymatic assay
  —Includes all reagents and buffers to run assay
  —No fluorescent or radioactive detection required


• 3 Simple Steps

• —Prepare enzyme mix
  —Add sample
  —Read Plate


• Product Background

• In this enzyme coupled assay, sphingomyelinase catalyzes the hydrolysis of sphingomyelin into phosphorylcholine and ceramide. Alkaline phosphatase catalyzes the hydrolysis of phosphorylcholine and the oxidation of choline to hydrogen peroxide is catalyzed by choline oxidase. DAOS plus 4-Aminoantipyrine (4 AAP) in the presence of hydrogen peroxide and peroxidase results in the oxidative coupling of DAOS and 4 AAP to form a blue chromogen that is detected by measuring the absorbance of light at 595 nm. Experiments have shown the Sphingomyelinase Assay Kit can detect sphingomyelin at concentrations as small as 78 μU/mL in 4 hours. The kit provides all necessary reagents to measure the sphingomyelinase activity of 40 samples ran in duplicate.

Storage

• -20°C
• Sphingomyelinase Assay Kit
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