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- ATCC
- 美国/中国
- xy-3600
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4℃保存
- 保质期：
6个月
- 英文名：
PI(3)P Mass Strip Kit
- 库存：
1
- 供应商：
上海信裕
- PI(3)P Mass Strip Kit详细信息：
-
Catalog No.: xy-3600
Description: The PI(3)P Mass Strip Kit measures the amount of PI(3)P obtained from cell extractions through a simple protein-lipid overlay assay.The PI(3)P Mass Strip kit is designed to quantify PI(3)P obtained from cell extractions through a simple lipid-protein overlay experiment. After following a non-radioactive lipid extraction protocol, the PI(3) P samples obtained from millions of cells are spotted onto a nitrocellulose strip which has been pre-spotted with PI(3)P standards and PIPn controls. Each strip has space for spotting 8 unknown cell extraction samples.
PI(3)P Mass Strip KitKit Contents
—PI(3)P Strips
—PI(3)P Detector
—Secondary Detector
Customer Feedback:
- "We thank you for your help and support with the PI(3)P mass strip assay and could show that results obtained with this assay were similar to those obtained with tritiated myo-inositiol labeling and HPLC." Angela Wandinger-Ness (University of New Mexico School of Medicine)
Reference
- Nonradioactive Quantification of Phosphoinositide 3-kinase (PI3K) Products from Breast Cancer Cell Lines andQuantification of PI3-Kinase Products from Breast Cancer Cell Lines
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- "We thank you for your help and support with the PI(3)P mass strip assay and could show that results obtained with this assay were similar to those obtained with tritiated myo-inositiol labeling and HPLC." Angela Wandinger-Ness (University of New Mexico School of Medicine)
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文献和实验【转帖】2-D Clean-Up Kit 双向凝胶电泳--蛋白纯化
System (GE Healthcare). After separation in the first dimension, the strip is run on the second dimension and the gels are either commassie or silver stained. The contents of the kit are sufficient for 50 purifications. The kit is stable
Determining In Vivo Phosphorylation Sites Using Mass Spectrometry
. Am. Soc. Mass Spectrom. 10:35‐44. Beausoleil, S.A., Jedrychowski, M., Schwartz, D., Elias, J.E., Villen, J., Li, J., Cohn, M.A., Cantley, L.C., and Gygi, S.P. 2004. Large‐scale
on ice for 30 min (all antibodies 1/100 v/v). 4. Wash with 10 mL of PBS/FBS, spin and resuspend in PBS/FBS with 1 µg/mL of propidium iodide. Filter through 70 µm nylon mesh. 5. Select cells which are PI negative, c-kit positive, Sca-1 positive
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