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F17a-A/F17a-A蛋白Recombinant Esc

herichia coli F17 fimbrial protein (F17a-A)重组蛋白F17 fimbrial adhesive antigen F17 pilin蛋白
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  • ¥2328 - 14796
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  • CSB-EP318126ENL
  • 2025年07月14日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

    • 保质期

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 英文名

      Recombinant Escherichia coli F17 fimbrial protein (F17a-A)

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      1mg/100μg/20μg

    规格:1mg产品价格:¥14796.0
    规格:100μg产品价格:¥4374.0
    规格:20μg产品价格:¥2328.0

    纯度:

    Greater than 90% as determined by SDS-PAGE.

    基因名:

    F17a-A

    Uniprot No.:

    P11312

    别名:

    F17 fimbrial adhesive antigen F17 pilin

    种属:

    Escherichia coli

    蛋白长度:

    Full Length of Mature Protein

    来源:

    E.coli

    分子量:

    32.4 kDa

    表达区域:

    22-180aa

    氨基酸序列:

    YDGKITFNGKVVDQTCSVTTESKNLTVKLPTVSANSLASSGKVVGLTPFTILLEGCNTPAVTGAQNVNAYFEPNANTDYTTGNLTNTASSGASNVQIQLLNADGVKAIKLGQAAAAQSVDTVAINDANVTLRYNAQYYATGVATAGDVTSTVNYTIAYQ

    蛋白标签:

    N-terminal 6xHis-SUMO-tagged

    产品提供形式:

    Liquid or Lyophilized powder

    缓冲液:

    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

    货期:

    3-7 business days

    注意事项:

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

    功能:

    Fimbriae (also called pili), polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell, enable bacteria to colonize the epithelium of specific host organs.

    内毒素:

    Not test.

    SDS-PAGE:

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    LC-MS Image Description/Western Blot:

    /

    Product types:

    In Stock Protein

    Biological_Activity:

    /

    Research Areas:

    Microbiology

    Reconstitution:

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    Reference:

    "Isolation and nucleotide sequence of the F17-A gene encoding the structural protein of the F17 fimbriae in bovine enterotoxigenic Escherichia coli."Lintermans P., Pohl P., Deboeck F., Bertels A., Schlicker C., Vandekerckhove J., van Damme J., van Montagu M., de Greve H.Infect. Immun. 56:1475-1484(1988)

    Function:

    Fimbriae (also called pili), polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell, enable bacteria to colonize the epithelium of specific host organs.

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    图标文献和实验
    该产品被引用文献
    "Isolation and nucleotide sequence of the F17-A gene encoding the structural protein of the F17 fimbriae in bovine enterotoxigenic Escherichia coli."Lintermans P., Pohl P., Deboeck F., Bertels A., Schlicker C., Vandekerckhove J., van Damme J., van Montagu M., de Greve H.Infect. Immun. 56:1475-1484(1988)
    相关实验
    • Strategies to Optimize Protein Expression in E. coli

         Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339

    • 单词 F

      Figure eight (8字型):由尚未完成的重组生成的两个相互连接的环状DNA。 Filter hybridization (滤膜杂交):将变性DNA样本固定于硝酸纤维膜上,然后用放射性标记 的DNA 或RNA 进行杂交。 Fingerprint of DNA (DNA 指纹图谱):指不同基因组间的不同多形态限制性片段模式。 Fingerprint of protein (蛋白质指纹图谱):酶(如胰蛋白酶)切割蛋白质后产生的片段模式(通 常用双向凝胶电泳分离)。

    • Construction of Long Chromosomal Deletion Mutants of Escherichia coli and Minimization of the Genome

      essential genetic information through experimental reduction of the genome to its minimal gene set. Taking advantage of recent technical advances, we constructed sequence-specific long deletion mutations of the Escherichia coli chromosome. In a recent report

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    文献支持
    F17a-A/F17a-A蛋白Recombinant Escherichia coli F17 fimbrial protein (F17a-A)重组蛋白F17 fimbrial adhesive antigen F17 pilin蛋白
    ¥2328 - 14796