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RNA Polymerase II Monoclonal Antibody Sampler
1
上海信裕
Description | A sampler pack of our three RNA Polymerase II Monoclonal Antibodies | |
Clone | 8WG16 (IgG2a), H5 (IgM), H14 (IgM) | |
Form | Ascites | |
Host | Mouse | |
Species Reactivity | RNA Polymerase II Monoclonal Antibody Sampler Extensive (Yeast to Human) | |
Specificity | The antibody 8WG16 was developed using RNA polymerase (RNA pol II) purified from wheat germ extract. It recognizes the C-terminal heptapeptide repeat present on the largest subunit of pol II. Clone 8WG16 has been used to investigate the role of the C-terminal domain of the 220kD RNA pol II subunit in the formation of the transcription complex. The antibody H5 was developed against a purified phosphorylated form of RNA polymerase II extracted from a transformed cell line. Pol II has two physiologically important phosphorylation sites; ser-2 and ser-5. Both sites are found in the heptapeptide repeat YSPTSPS at the C-terminal domain. This antibody recognizes the phosphoserine 2 version of pol II. The antibody H14 was developed against a purified phosphorylated form of RNA polymerase II extracted from a transformed cell line. Pol II has two physiologically important phosphorylation sites; ser-2 and ser-5. Both sites are found in the heptapeptide repeat YSPTSPS at the C-terminal domain. This antibody recognizes the phosphoserine 5 version of pol II. |
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Uses | These antibodies are effective in immunoblotting, immunofluorescence (IF), immunoprecipitation (IP), pol II purification and functional studies. | |
Suggested Working Dilution | Quality control tests were done by Western blot and Dot blot. The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval.
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Notes | RNA Polymerase II Monoclonal Antibody Sampler Predicted MW = 220-240 kD *use casein or BSA in the blocking solution rather than non-fat-dry milk |
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Storage | Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. | |
References | SH Ahn, M Kim, S Buratowski. Phosphorylation of Serine 2 with the RNA Polymerase II C-Terminal Domain Couples Transcription and 3’ End Processing. Mol. Cell 13:67-76, 2004. JM Espinosa, RE Verdun, BM Emerson. p53 functions through stress- and promoter-specific recruitment of transcription initiation components before and after DNA damage. Mol. Cell. 12(4):1015-27, 2003. L Pellizzoni, B Charroux, J Rappsilber, M Mann, G Dreyfuss. A functional interaction between the survival motor neuron complex and RNA polymerase II. J. Cell. Biol. 152(1):75-85, 2001. SR Bhaumik, MR Green. SAGA is an essential in vivo target of the yeast acidic activator Gal4p. Genes. Dev. 15(15):1935-1945, 2001. M Patturajan, RJ Schulte, BM Sefton, R Berezney, M Vincent, O Bensaude, SL Warren, JL Corden. Growth-related changes in phosphorylation of yeast RNA polymerase II. J. Biol. Chem. 273(8):4689-4694, 1998. DB Bregman, L Du, S van der Zee, SL Warren. Transcription-dependent redistribution of the large subunit of RNA polymerase II to discrete nuclear domains. J. Cell. Biol. 129:287-298, 1995. J Zhang, JF Corden. Identification of phosphorylation sites in the repetitive carboxyl-terminal domain of the mouse RNA polymerase II largest subunits. J. Biol. Chem. 266:2290-2296, 1991. NE Thompson, TH Steinberg, DB Aronson, RR Burgess. Inhibition of in-vivo and in-vitro transcription by monoclonal antibodies prepared against wheat germ RNA polymerase II that react with the heptapeptide repeat of eukaryotic RNA polymerase II. J. Biol. Chem. 264:11511-11520, 1989. |
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Warranty/Conditions | Covance products may not be resold or modified for resale without prior written approval. | |
Rev. Date | 5/23/2007 RNA Polymerase II Monoclonal Antibody Sampler |
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