TRI Reagent® T9424-25ML

产品名称
Frequently Asked QuestionsLive Chat andFrequently Asked Questions are available for this Product.ApplicationTRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski.1The RNA isolation method based on this reagent is widely used and proven for RNA applications2. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.The resulting RNA can be used for Northern blots, mRNA isolation,in vitrotranslation, RNase protection assay, cloning, and PCR.Features and Benefits• Easily scalable RNA isolation
• Works with many sources: human, plant, yeast, bacterial, or viral
• Better yields than traditional guanidine thiocyanate/cesium chloride methods
包装25, 100, 200 mL in glass bottleGeneral descriptionTRI Reagent is a quick and convenient reagent for use in the simultaneous isolation of RNA, DNA, and protein. Successful isolations from human, animal, plant, yeast, bacterial, and viral samples can be obtained. A convenient single-step liquid phase separation results in the simultaneous isolation of RNA, DNA, and protein. This procedure is an improvement of the single-step method reported by Chomczynski and Sacchi for total RNA isolation. TRI Reagent performs well with large or small amounts of tissue or cells and many samples can be simultaneously extracted.

This product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effectively dissolves DNA, RNA, and protein on homogenization or lysis of tissue sample. After adding chloroform or 1-bromo-3-chloropropane and centrifuging, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each component can then be isolated after separating the phases. One ml of TRI Reagent is sufficient to isolate RNA, DNA, and protein from 50-100 mg of tissue, 5-10 ´ 106 cells, or 10 cm2 of culture dish surface for cells grown in monolayer.

This is one of the most effective methods for isolating total RNA and can be completed in only 1 hour starting with fresh tissue or cells. The procedure is very effective for isolating RNA molecules of all types from 0.1-15 kb in length. The resulting RNA is intact with little or no contaminating DNA and protein.法律信息TRI Reagent 为以下机构的注册商标： Molecular Research Center, Inc.
性质
Related CategoriesCore Bioreagents,DNA和RNA纯化,General Reagents,General Stem Cell Biology,Life Science Reagents for Nucleic Acid Purification,
Plant Biotechnology,RNA Purification,RNA/DNA Isolation Products for Stem Cell Biology,RNA纯化,Research Essentials,Stem Cell Characterization,分子生物学,干细胞生物学,植物分子生物学,植物核酸纯化,细胞生物学更多...usage mL sufficient for 107 cells mL sufficient for 100 mg tissue (or)