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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°-20°C
- 保质期:
1-3年
- 库存:
999
- 供应商:
亦高生物
- 规格:
T9424-200ML

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文献和实验RNA purification using TRizol--TRIZOL法提取RNA
Reagents and EquipmentsTRIzol Reagent (Life Technologies cat# 15596-026) or TRI reagent (Sigma cat # T-9424)DEPC (RNase free) water or 0.5% SDS solution in DEPC treated waterChloroform (Fisher )Isopropyl alcohol (2-Propanol) (Fisher)75% Ethanol
How to Make Simple Solutions and Dilutions
volume needed = mass of reagent to use. Example 1: If you want to make 200 ml of 3 NaCl you would dissolve 0.03 g/ml x 200 ml = 6.0 g NaCl in 200 ml water. When using liquid reagents the percent concentration is based
PREPARATION OF STOCK SOLUTIONS FOR FISH
17.53 g NaCl, 8.82 g tri-Na-citrate and 5.68 g Na2HPO4 in 200 ml water (10xSSCP) -mix 300 ml formamide with 100 ml 10xSSCP and 100 ml water, bring to pH 7.0 with HCl 1.0 M Tris + 1.5 M NaCl pH 7.5 -for 5 l take 605.7 g Tris and 438.3 g NaCl
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