多功能干细胞神经诱导培养基 PSCNIM 无血清

Sciencell公司产品由上海中乔新舟生物科技有限公司优质供应

美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力
于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养
基的研究和开发，在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高
的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。

ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21
种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。
确保了实验结果的重复性和连贯性。

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Human embryonic stem cells (hESC) and induced pluripotent stem cells (hiPSC) possess the capability of differentiating into
all derivatives of the three germ layers including neural cells. Human pluripotent stem cell (hPSC) derived neural cells have
been used for studying nervous system development, modeling neurologic disorders, neurotoxicology and drug screening.
SMAD signaling plays a crucial role in neural induction. Dual inhibition of SMAD signaling by Noggin and SB431542 is sufficient
to induce rapid and complete neural conversion of hPSCs [1]. In 2010, Wenlin Li and colleagues found that synergistic inhibition
of glycogen synthase kinase 3 (GSK3) and transforming growth factor β (TGF-β) could efficiently differentiate hPSCs to
homogenous neural epithelium in 7 days [2]. The derived neural stem cells (NSCs) can self-renew in the presence of human
leukemia inhibitory factor (LIF) and inhibitors of GSK3 and TGF-β. NSCs possess the multipotential of differentiating into various
neuronal and glial subtypes upon induction with specific patterning cues [2].

Based on the advances in neural differentiation methodologies, we developed hPSC Neural Induction Medium
(PSCNIM, Cat. #5931), a serum-free medium, to efficiently convert hPSCs to NSCs under adherent culture conditions. The hPSCs
are converted to homogenous neural epithelium using neural induction medium (Figure 1). After a 7 – 10 day treatment with
PSCNIM, more than 90% of cells expressed the neural stem cell markers: Nestin and Sox2; while the pluripotency marker Nanog
expression was lost (Figure 2). NSCs derived from hPSC can be maintained in the induction medium for at least 5 passages
(~24 days), can efficiently form rosettes (Figure 3) and be expanded as neural progenitor cells in EGF and FGF containing medium,
or differentiated into neural lineages such as neurons and astrocytes (Figure 3).