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Recombinant Human MHC class I chain-related gene A
1.当细胞铺满整个平皿(100mm)后,吸弃旧培养液,10mLPBS洗一遍
2.0.25%胰酶(预热20分钟左右)2ml消化,轻轻摇晃,Recombinant Human MHC class I chain-related gene A直至肉眼见单层细胞呈块状脱落(或在显微镜下观察细胞之间分离),立刻加5ml(DMEM+10%FBS)终止消化,轻柔吹打8-10次成单细胞。
3.离心1000rpm*5min,弃上清,加DMEM+10%FBS轻柔吹打。
4.将细胞悬液1:4传到100mm细胞培养皿中,每个皿加10ml(DMEM+10%FBS)培养液,正常情况下2-3天就可以长满,期间不用换液。
Recombinant Human MHC class I chain-related gene A的传代方法:首先在倒置显微镜下观察生长融合达80%,不需要长满,就准备传代。倒掉旧的培养基,再用已经高压灭菌过的PBS洗1-2次,我用的是25平方厘米的培养瓶,加入1毫升已经配制好的0.25%胰蛋白酶-0.02%EDTA(0.25%胰蛋白酶是用D-Hanks配制后先用滤纸过滤,再用一次性过滤器过滤除菌,Recombinant Human MHC class I chain-related gene A再分装成1ml的小包装,刚好每次用完一个,避免每次反复冻存,会使胰酶的活性下降),加入的量以刚好盖满瓶底为宜。放到倒置显微镜下观察变化,一旦发现细胞开始变园收缩,大约1-3分钟时间,必要时可以吹打帮助细胞分散,就立即加入培养基中和胰酶,如有EDTA最好要离心(800rpm,5min),弃上清夜,Recombinant Human MHC class I chain-related gene A再加入完全培养基吹匀,再分瓶,一般一瓶可以分2-3瓶。放入5%的CO2培养箱,37度培养24小时后观察。
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文献和实验Molecular Typing of the MHC Class I Chain-Related Gene Locus
The human major histocompatibility complex (MHC) class I chain-related genes A and B (MICA and MICB ) are located within the human leukocyte antigen (HLA) class I region of chromosome 6 (1 ). Their organization, expression, and products
in immunotherapy because of their highly restricted fine specificity. Expression of specific peptides in complex with MHC class I molecules on cells was shown to be associated with cancer and autoimmune disorders (1 –8 ). For example, human melanomas express
In Vitro Reconstitution of the MHC Class I Peptide-Loading Complex
involved in general ER glycoprotein folding, to bridge and thereby stabilize its otherwise weak interaction with the MHC class I heavy chain. Here, we describe methods for the recombinant expression of soluble components of the PLC specifically tailored
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