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Human IL-12

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  • ¥1200 - 19200
  • SinoBio
  • 国产
  • CI58
  • 2025年11月08日
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    • 文献和实验
    • 技术资料
    • 保存条件

      蛋白表达区间:Arg23-Ser219&Ile23-Ser328

    • 保质期

      See instructions

    • 英文名

      标签:Tag free

    • 库存

      表达系统:Human Cells

    • 供应商

      上海经科化学科技有限公司

    • 规格

      10ug/50ug/500ug/1mg

    规格:10ug产品价格:¥1200.0
    规格:50ug产品价格:¥3520.0
    规格:500ug产品价格:¥12320.0
    规格:1mg产品价格:¥19200.0
    产品描述
    Recombinant Human Interleukin-12 is produced by our Mammalian expression system and the target gene encoding Arg23-Ser219&Ile23-Ser328 is expressed.
     
    蛋白编号
    P29459&
    P29460
     
    产品别称
    Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell; IL12A ; NKSF1 stimulatory factor chain 1
     
    分子量
    22.5&34.7 KDa
     
    表观分子量
    25-38&40-50 KDa, reducing conditions
     
    剂型描述
    Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
     
    内毒素
    < 1 EU/µg as determined by LAL test.
     
    纯度-SDS-PAGE
    Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
     
    纯度-SEC-HPLC
    Greater than 95% as determined by SEC-HPLC. (QC verified)
     
    复溶
    • Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
    • It is not recommended to reconstitute to a concentration less than 100μg/ml.
    • Dissolve the lyophilized protein in distilled water.
    • Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 
     
    运输条件
    • The product is shipped at ambient temperature.
    • Upon receipt, store it immediately at the temperature listed below.
     
    保存条件
    • Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
    • Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
    • Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
     
    产品背景
    IL-12 is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit.Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity.IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes.

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    图标文献和实验
    该产品被引用文献

    IL-12 is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit.Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity.IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes.

    相关实验
    • IL-12相关问题

      mapan777:不知道哪位能告诉我IL-12IL-12p40、IL-12p35、IL-12p70之间的关系是什么啊?丁香园网友qdwanfeng2007认为:我也正想研究il-12,希望能和你共同探讨一下,P40和P35是P70的两个亚基吧?丁香园网友mapan777认为:是的,它们联合起来起作用,我想做PCR,但是快被这个引物折磨死了,不知楼上是要做什么?丁香园网友qdwanfeng2007认为:我想研究研究白介素12的抗肿瘤作用和机理,没大研究过PCR,可能帮不了你。是不是引物

    • 小鼠白细胞介素-12(IL-12)酶联免疫分析(ELISA)

      小鼠白细胞介素-12(IL-12) 酶联免疫 分析( ELISA ) 试剂 盒使用说明书 本试剂仅供研究使用          目的:本试剂盒用于测定小鼠血清,血浆及相关液体样本中白细胞介素-12(IL-12)含量。 实验原理 :       本试剂盒应用双抗体夹心法测定 标本 中 小鼠白细胞介素-12 ( IL-12 ) 水平。用纯化的 小鼠白细胞介素-12 ( IL-12 ) 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 IL-12

    • 猴子白细胞介素12(IL-12)酶联免疫分析(ELISA)

      猴子 白细胞介素12(IL-12) 酶联免疫 分析( ELISA ) 试剂 盒使用说明书 本试剂仅供研究使用          目的:本试剂盒用于测定猴子血清,血浆,细胞上清及相关液体样本中 白细胞介素12(IL-12)的 含量。 实验原理 :    本试剂盒应用双抗体夹心法测定 标本 中 猴子 白细胞介素12 ( IL-12 ) 水平。用纯化的 猴子 白细胞介素12 ( IL-12 ) 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次

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    文献支持
    Human IL-12
    ¥1200 - 19200