Human TNF RII(C830)产品图
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Human TNF RII(C830)

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  • ¥840 - 17600
  • SinoBio
  • 国产
  • C830
  • 2026年04月28日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件:

      蛋白表达区间:Pro24-Thr206

    • 保质期:

      See instructions

    • 英文名:

      标签:C-mFc

    • 库存:

      表达系统:Human Cells

    • 供应商:

      上海经科化学科技有限公司

    • 规格:

      10ug/50ug/500ug/1mg

    规格:10ug产品价格:¥840.0
    规格:50ug产品价格:¥2520.0
    规格:500ug产品价格:¥12320.0
    规格:1mg产品价格:¥17600.0
    产品描述
    Recombinant Human Tumor Necrosis Factor Receptor Superfamily Member 1B is produced by our Mammalian expression system and the target gene encoding Pro24-Thr206 is expressed with a mouse IgG1 Fc tag at the C-terminus.
    蛋白编号
    P20333
    产品别称
    Tumor necrosis factor receptor superfamily member 1B; TNFRSF1B; Tumor necrosis factor receptor 2; TNF-R2; TNF-RII; Tumor necrosis factor receptor type II; p75; p80 TNF-alpha receptor; CD120b
    偶联
    Unconjugated
    分子量
    46.44 KDa
    表观分子量
    60 KDa, reducing conditions
    剂型描述
    Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
    内毒素
    < 1 EU/µg as determined by LAL test.
    纯度-SDS-PAGE
    Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
    生物活性-ELISA
    Immobilized Recombinant Human TNF RII-mFc(Cat#C830) at 1μg/ml (100 μl/well) can bind Recombinant Human TNF alpha-His(Cat#CG91). The ED50 of Recombinant Human TNF alpha-His(Cat#CG91) is 1.97 ng/ml. (Regularly tested)
    生物活性-BLI
    Loaded Human TNF alpha-His(Cat#CG91) on HIS1K Biosensor, can bind Human TNF RII-mFc(Cat#C830) with an affinity constant of 1.83 nM as determined in BLI assay. (Regularly tested) 
    复溶
    • Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
    • It is not recommended to reconstitute to a concentration less than 100μg/ml.
    • Dissolve the lyophilized protein in distilled water.
    • Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 
    运输条件
    • The product is shipped at ambient temperature.
    • Upon receipt, store it immediately at the temperature listed below.
    保存条件
    • Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
    • Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
    • Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
    产品背景
    Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) is a member of the tumor necrosis factor receptor superfamily. Human TNF RII contains four cysteinerich repeats in its ECD, which shares 58% and 56% amino acid sequence identity with the mouse and rat orthologs, respectively.TNF RII is expressed predominantly on cells of the hematopoietic lineage, such as T and natural killer cells, as well as on endothelial cells, microglia, astrocytes,neurons, oligodendrocytes, cardiac myocytes, thymocytes, and mesenchymal stem cells.TNF RII binds to the membranebound forms of TNFα and Lymphotoxinα/TNFβ;soluble TNF is thought to signal predominately through TNF RI.Soluble TNF RII is believed to inhibit TNF biological activity by binding TNF thereby preventing it from activating membrane TNF receptors.

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    图标文献和实验
    该产品被引用文献

    Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) is a member of the tumor necrosis factor receptor superfamily. Human TNF RII contains four cysteinerich repeats in its ECD, which shares 58% and 56% amino acid sequence identity with the mouse and rat orthologs, respectively.TNF RII is expressed predominantly on cells of the hematopoietic lineage, such as T and natural killer cells, as well as on endothelial cells, microglia, astrocytes,neurons, oligodendrocytes, cardiac myocytes, thymocytes, and mesenchymal stem cells.TNF RII binds to the membranebound forms of TNFα and Lymphotoxinα/TNFβ;soluble TNF is thought to signal predominately through TNF RI.Soluble TNF RII is believed to inhibit TNF biological activity by binding TNF thereby preventing it from activating membrane TNF receptors.

    相关实验
    • Production and Characterization of Recombinant Human and Murine TNF

      takes about 2 wk. Human and murine TNF can be purified from crude extracts with high yields (>50 mg from 1 L of fermentation) by hydrophobic-interaction chromatography, ion-exchange chromatography, and gel-filtration chromatography. The purity

    • Mutational Analysis of the Human Protein C Gene

      The single gene for protein C is located at position q13–q14 on chromosome 2 (1 ). Two groups have described human genomic clones of protein C isolated from phage l charon libraries using cDNA for human protein C as hybridization probes

    • Detection of TNF and TNF Receptor mRNA in Cells and Tissues

      We describe a semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) technique for the quantification of the messenger RNA of human and murine tumor necrosis factor α (TNFα) and related receptors. This protocol can be adapted

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    文献支持
    Human TNF RII(C830)
    ¥840 - 17600