The EpiQuik™ Acetyl-Histone H4 ChIP Kit is a convenient package of tools that allows the experimenter to investigate interactions of histone H4 acetylation and DNA in vivo efficiently. The entire procedure can be completed within 5 hours and produces far superior results than any competitor kits. The EpiQuik™ Acetyl-Histone H4 ChIP kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot as well as DNA microarray.
WHY CHOOSE THE EPIQUIK™ ACETYL-HISTONE H4 ChIP KIT?
The fastest procedure available, which can be finished within 5 hours.
Strip microwell format makes the assay flexible: manual or high throughput.
Columns for DNA purification are included: save time and reduce labor.
Compatible with all DNA amplification-based approaches.
Simple, reliable, and consistent assay conditions.
乙酰化组蛋白H4免疫沉淀试剂盒Principle & Procedure
The EpiQuik™ Acetyl-Histone H4 ChIP kit contains all reagents required for carrying out a successful chromatin immunoprecipitation for acetyl-histone H4 from mammalian cells. Particularly, this kit includes a ChIP-grade acetyl-histone H4 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured acetyl-histone H4protein-DNA complex, reversed and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.