- 询价
- 美国eBioscince
- 美国/中国
- xy-1016
- 2025年07月10日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4℃保存
- 保质期：
6个月
- 英文名：
Methylamp Whole Cell Bisulfite Modification Kit
- 库存：
1
- 供应商：
上海信裕
- 全细胞亚硫酸盐修饰试剂盒详细信息：
-
Product Overview
The Methylamp™ Whole Cell Bisulfite Modification Kit is an innovative and unique set of essential components which enables the experimenter to perform DNA methylation analysis and modify DNA directly from cells or tissues using Epigenteks uniquely simplified and streamlined bisulfite method. The entire procedure can be completed within only 3 hours. The Methylamp™ Whole Cell Bisulfite Modification Kit is specifically designed for DNA methylation research using minute amounts of starting materials including cells cultured in 96-well/384 well plates, tissue section samples, microdissection samples, tissue biopsy and early embryonic cells/oocytes.
Eluted modified DNA by using the Methylamp™ Whole Cell Bisulfite Modification Kit is suited for real time MS-PCR. It is also suitable for all techniques currently used for the analysis of DNA methylation; including conventional MS-PCR, bisulfite sequencing, pyrosequencing, and methylation microarray. If you use the Methylamp™ Whole Cell Bisulfite Modification Kit for MSP, the numbers of PCR cycles should be greater than 45. The amount of starting materials for each modification can be 100-20000 cells, or 1 µg-100 µg tissues, or 0.2-2 mm2 tissue section samples. For optimal modification, the amount should be 500- 5000 cells, or 5-20 µg tissues, or 0.5-1 mm2 tissue section samples, respectively.
WHY CHOOSE THE METHYLAMP™ DNA MODIFICATION KIT?- Proprietary and unique by directly modifying cells and tissues.
- Rapid streamlined 3 hour procedure from cells/tissues to modified DNA.
- Completely converts unmethylated cytosine into uracil: modified DNA > 99.5%.
- The lowest degradation of DNA in the modification process: more than 90% of DNA loss can be prevented with a unique DNA protection buffer.
全细胞亚硫酸盐修饰试剂盒Principle & Procedure
The Methylamp™ Whole Cell Bisulfite Modification Kit contains all reagents required for bisulfite conversion directly on a cell or tissue sample. The kit allows DNA to be isolated from cells or tissues, denatured and bisulfite modified simultaneously in same tube with the specific reaction buffer under the thermodynamic condition. In the modification process, bisulfite reagent reacts specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. The unique DNA protection reagents contained in the modification buffer can prevent the chemical and thermophilic degradation of DNA in the bisulfite treatment. The non-toxic modified DNA capture buffer enables DNA to tightly bind to the column filter, thus DNA cleaning can be carried out on the column to effectively remove residual sodium bisulfite and salts. Modified DNA can then be eluted and stably stored at –20°C for up to 2 months.
SCHEMATIC PROCEDURE: 
The different amounts of MCF-7 cells or DNA isolated from MCF-7 cells were modified using the Methylamp™ Whole Cell Bisulfite Modification Kit or Methylamp™ One-Step DNA modification kit, respectively. 10 µl of modified DNA were eluted and 2 µl of elution were used in real time PCR. A pair of primers and a probe designed to amplify both methylated and unmethylated alleles of β-actin was used.全细胞亚硫酸盐修饰试剂盒Product Components
W1 (Digestion powder)
W2 (Digestion solution)
W3 (Cell collection buffer)
W4 (DNA modification powder)
W5 (DNA modification buffer)
W6 (Balance buffer)
W7 (DNA binding buffer)
W8 (modified DNA elution)
F-spin column
F-collection tube
User guide
51210-50 超快核酸电泳液
3140-1.5 RNA电泳带锐化液
90313-250 TBE电泳缓冲液，10×
100211-250 TAE缓冲液，50×
100211-2500 TAE缓冲液，50×
3150-100 RNA电泳缓冲液，10×
100816-250 碱性缓冲液，10×
100909-200 长效SDS-PAGE配胶液
100910-10 SDS-PAGE专用还原剂
100911-1 长效SDS-PAGE上样液
100912A-20 长效SDS-PAGE专用电泳液（干粉）
100912B-20 长效SDS-PAGE专用电泳液（干粉）
100873-500 尿素（电泳级）干粉
100874-1 尿素变性PAGE上样液，6×
100874-10 尿素变性PAGE上样液，6×
100875-1 核酸非变性PAGE上样液, 6×
100875-10 核酸非变性PAGE上样液, 6×
3690A DNA电泳上样液
3690B DNA电泳上样液
3130A-1.5 三合一RNA上样液
3130B-1.5 三合一RNA上样液
70608-1.5 miRNA电泳上样液
70608-10 miRNA电泳上样液
3280-50 电泳级SYBR Green I
61201-50 电泳级耐热型SYBR染料
70303-0.5 绿如蓝核酸染料
70303-1.5 绿如蓝核酸染料
81104-1000 超快核酸银染试剂盒
100603-30 银染清除剂
3092-100 溴化乙锭清除剂
100808-1 溴化乙锭干粉
3180-1.5 溴化乙锭溶液
70503 DNA电泳分子量标准系列
90602 DNA电泳分子量标准系列（2）
90101-50 大片段DNA分子量标准
70605-30 miRNA电泳分子量标准全细胞亚硫酸盐修饰试剂盒User Guide & MSDS
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人缺血修饰白蛋白 (IMA)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 IMA 单抗包被于酶标板上，标准品和样品中的 IMA与单抗结合，加入生物素化的抗人 IMA ，形成
LightCycler通过实时荧光PCR技术进行DNA甲基化分析
的定量。 更多有关特定CpG位点的甲基化的详细信息，可采用重亚硫酸氢盐修饰后测序分析。 LightCycler主要应用：实时荧光PCR技术进行快速准确的DNA甲基化分析 我们使用罗氏诊断公司的LightCycler®480高分辨熔解扩增试剂盒在LightCycler®480实时荧光PCR仪上，通过MS-HRM测定法对两种已知的经过启动子（FANCE和MGMT）甲基化的DNA修复基因的检测性能。 材料和方法 将多种细胞株的DNA样本作为检测模板。每μg的每种DNA样本都经过重亚硫酸盐修饰
引物（红色星号=突变核苷酸，灰色线条 = 删除的序列，蓝色线条 = 插入的序列）。也可考虑使用其他引物设计，如具有 5′ 重叠序列的引物[4-6]。 图 6.使用含突变序列和同源末端序列的 PCR 引物进行的定点突变。该图所示方法阐述了 Invitrogen™ GeneArt™ 定点突变试剂盒的机制，其中，方块代表重组和突变位点。 5.甲基化 PCR 可用于研究位点特异性甲基化。在 甲基化特异性 PCR（MSP）方法中，设计了两个引物对，以区分目标位点的甲基化状态[7,8]。 首先使用重亚硫酸盐处理
技术资料暂无技术资料 索取技术资料
