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- ZK-0019544
- 美国/中国
- 2025年11月20日
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- 详细信息
- 技术资料
- 库存：
1
- 英文名：
Methylamp Whole Cell Bisulfite Modification Kit
- 保质期：
6个月
- 供应商：
上海再康
- 保存条件：
4℃保存
- 全细胞亚硫酸盐修饰试剂盒详细信息：
-
Product Overview
The Methylamp™ Whole Cell Bisulfite Modification Kit is an innovative and unique set of essential components which enables the experimenter to perform DNA methylation analysis and modify DNA directly from cells or tissues using Epigenteks uniquely simplified and streamlined bisulfite method. The entire procedure can be completed within only 3 hours. The Methylamp™ Whole Cell Bisulfite Modification Kit is specifically designed for DNA methylation research using minute amounts of starting materials including cells cultured in 96-well/384 well plates, tissue section samples, microdissection samples, tissue biopsy and early embryonic cells/oocytes.
Eluted modified DNA by using the Methylamp™ Whole Cell Bisulfite Modification Kit is suited for real time MS-PCR. It is also suitable for all techniques currently used for the analysis of DNA methylation; including conventional MS-PCR, bisulfite sequencing, pyrosequencing, and methylation microarray. If you use the Methylamp™ Whole Cell Bisulfite Modification Kit for MSP, the numbers of PCR cycles should be greater than 45. The amount of starting materials for each modification can be 100-20000 cells, or 1 µg-100 µg tissues, or 0.2-2 mm2 tissue section samples. For optimal modification, the amount should be 500- 5000 cells, or 5-20 µg tissues, or 0.5-1 mm2 tissue section samples, respectively.
WHY CHOOSE THE METHYLAMP™ DNA MODIFICATION KIT?- Proprietary and unique by directly modifying cells and tissues.
- Rapid streamlined 3 hour procedure from cells/tissues to modified DNA.
- Completely converts unmethylated cytosine into uracil: modified DNA > 99.5%.
- The lowest degradation of DNA in the modification process: more than 90% of DNA loss can be prevented with a unique DNA protection buffer.
全细胞亚硫酸盐修饰试剂盒Principle & Procedure
The Methylamp™ Whole Cell Bisulfite Modification Kit contains all reagents required for bisulfite conversion directly on a cell or tissue sample. The kit allows DNA to be isolated from cells or tissues, denatured and bisulfite modified simultaneously in same tube with the specific reaction buffer under the thermodynamic condition. In the modification process, bisulfite reagent reacts specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. The unique DNA protection reagents contained in the modification buffer can prevent the chemical and thermophilic degradation of DNA in the bisulfite treatment. The non-toxic modified DNA capture buffer enables DNA to tightly bind to the column filter, thus DNA cleaning can be carried out on the column to effectively remove residual sodium bisulfite and salts. Modified DNA can then be eluted and stably stored at –20°C for up to 2 months.
SCHEMATIC PROCEDURE:
The different amounts of MCF-7 cells or DNA isolated from MCF-7 cells were modified using the Methylamp™ Whole Cell Bisulfite Modification Kit or Methylamp™ One-Step DNA modification kit, respectively. 10 µl of modified DNA were eluted and 2 µl of elution were used in real time PCR. A pair of primers and a probe designed to amplify both methylated and unmethylated alleles of β-actin was used.全细胞亚硫酸盐修饰试剂盒Product Components
W1 (Digestion powder)
W2 (Digestion solution)
W3 (Cell collection buffer)
W4 (DNA modification powder)
W5 (DNA modification buffer)
W6 (Balance buffer)
W7 (DNA binding buffer)
W8 (modified DNA elution)
F-spin column
F-collection tube
User guide
51210-50 超快核酸电泳液
3140-1.5 RNA电泳带锐化液
90313-250 TBE电泳缓冲液，10×
100211-250 TAE缓冲液，50×
100211-2500 TAE缓冲液，50×
3150-100 RNA电泳缓冲液，10×
100816-250 碱性缓冲液，10×
100909-200 长效SDS-PAGE配胶液
100910-10 SDS-PAGE专用还原剂
100911-1 长效SDS-PAGE上样液
100912A-20 长效SDS-PAGE专用电泳液（干粉）
100912B-20 长效SDS-PAGE专用电泳液（干粉）
100873-500 尿素（电泳级）干粉
100874-1 尿素变性PAGE上样液，6×
100874-10 尿素变性PAGE上样液，6×
100875-1 核酸非变性PAGE上样液, 6×
100875-10 核酸非变性PAGE上样液, 6×
3690A DNA电泳上样液
3690B DNA电泳上样液
3130A-1.5 三合一RNA上样液
3130B-1.5 三合一RNA上样液
70608-1.5 miRNA电泳上样液
70608-10 miRNA电泳上样液
3280-50 电泳级SYBR Green I
61201-50 电泳级耐热型SYBR染料
70303-0.5 绿如蓝核酸染料
70303-1.5 绿如蓝核酸染料
81104-1000 超快核酸银染试剂盒
100603-30 银染清除剂
3092-100 溴化乙锭清除剂
100808-1 溴化乙锭干粉
3180-1.5 溴化乙锭溶液
70503 DNA电泳分子量标准系列
90602 DNA电泳分子量标准系列（2）
90101-50 大片段DNA分子量标准
70605-30 miRNA电泳分子量标准全细胞亚硫酸盐修饰试剂盒User Guide & MSDS
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