人IL-3/Interleukin-3蛋白(His标签) 产品信息
别称:
IL-3, MCGF, MGC79398, MGC79399, MULTI-CSF
蛋白构建:
A DNA sequence encoding the human IL3 (NP_000579.2) (Met 1-Phe 152) was expressed, fused with a polyhistidine tag at the C-terminus.
种属:
Human
表达宿主:
Baculovirus-Insect Cells
人IL-3/Interleukin-3蛋白(His标签)产品状态&运输说明:
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
质控检测
纯度:
> 95% as determined by SDS-PAGE.
生物活性:
1. Measured in a cell proliferation assay using TF1 human erythroleukemic cells (Kitamura, T. et al. 1989 J. Cell Physiol. 140: 323.) . The ED50 for this effect is typically 0.3-1.5 ng/ml .
2. Measured by its binding ability in a functional ELISA . Immobilized human IL3 at 20 μg/ml (100 μl/well) can bind human IL3Ra with a linear ranger of 0.064-1.6 μg/ml. Immobilized human IL3 at 20 μg/ml (100 μl/well) can bind biotinylated human IL3Ra with a linear ranger of 0.32-1.6 μg/ml.
内毒素:
< 1.0 EU per μg of the protein as determined by the LAL method
稳定性:
Samples are stable for up to twelve months from date of receipt at -70℃
预测N端:
Ala 20
分子量:
The recombinant human IL3 consists of 143 amino acids and predicts a molecular mass of 16.5 kDa. It migrates as an approximately 19 kDa band in SDS-PAGE under reducing conditions.
人IL-3/Interleukin-3蛋白(His标签)缓冲液:
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
使用指导
储存方法:
Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶条件:
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.人IL-3/Interleukin-3蛋白(His标签) xy-hxbz-156 人非小细胞肺癌细胞 NCI-H838 规格25毫升/株.
xy-hxbz-157 人肺小细胞肺癌 NCI-H2227 规格25毫升/株.
xy-hxbz-158 人肺癌细胞(淋巴结转移) NCI-H292 规格25毫升/株.
xy-hxbz-159 人肺腺鳞癌细胞 NCI-H596 规格25毫升/株.
xy-hxbz-160 人非小细胞肺癌细胞 NCI-H23 规格25毫升/株.
xy-hxbz-161 人非小细胞肺腺癌细胞 NCI-H2087 规格25毫升/株.
xy-hxbz-162 人肺腺癌细胞 A549 规格25毫升/株.
xy-hxbz-163 人肺癌细胞 A-427 规格25毫升/株.
xy-hxbz-164 人肺癌细胞 H125 规格25毫升/株.
xy-hxbz-165 人肺巨细胞癌 PG 规格25毫升/株.
xy-hxbz-166 耐DDP肺癌细胞 A549/DDP 规格25毫升/株.
xy-hxbz-167 人肺扁平上皮癌细胞 QG-56 规格25毫升/株.
xy-hxbz-168 人高转移肺癌细胞 95-D 规格25毫升/株.
xy-hxbz-169 人巨细胞性肺癌细胞 801-D 规格25毫升/株.
xy-hxbz-170 人肺腺癌细胞 SPC-A1 规格25毫升/株.
xy-hxbz-171 人肺腺癌细胞 LTPEP-a-2 规格25毫升/株.
xy-hxbz-172 人肺腺癌细胞 LTEP-a-2 规格25毫升/株.
xy-hxbz-173 人肺腺癌细胞 H1299 规格25毫升/株.
xy-hxbz-174 人肺鳞癌细胞 SK-MES-1 规格25毫升/株.
xy-hxbz-175 人肺腺癌细胞(胸膜渗出液) Calu-3 规格25毫升/株.
xy-hxbz-176 人肺癌细胞系(未分化) Calu-6 规格25毫升/株.
xy-hxbz-177 人胚肺二倍体细胞 HLF-02 规格25毫升/株.
xy-hxbz-178 人肺癌细胞系 Tul-1 规格25毫升/株.
xy-hxbz-179 人胚肺成纤维细胞 HFL-I 规格25毫升/株.
xy-hxbz-180 人胚肺细胞 MRC-5 规格25毫升/株.
xy-hxbz-181 人胚肺细胞 WI-38 规格25毫升/株.
xy-hxbz-182 人SV-40转化肺成纤维细胞 WI-38AV13 规格25毫升/株.
xy-hxbz-183 人肺癌亚历山大细胞系 PLC/PRF/5 规格25毫升/株.
xy-hxbz-184 人肺巨细胞癌(PG)的低转移亚系 PG-LH7 规格25毫升/株.
xy-hxbz-185 人肺巨细胞癌(PG)的高转移亚系 PG-BE1 规格25毫升/株.
xy-hxbz-186 人肺腺癌细胞系 PG49 规格25毫升/株.
xy-hxbz-187 人肺腺癌细胞 GLC-82 规格25毫升/株.
编号 中文 英文名称 规格25毫升/株.
xy-hxbz-188 人肺巨细胞癌细胞 HLAmP 规格25毫升/株.