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- N1891-5SET
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温
- 库存：
现货
- 供应商：
上海善然生物科技有限公司
Application
Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each Sigma FAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST™ require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.
Reconstitution
Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing pNPP.
法律信息
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
性质
Related Categories p-Nitrophenyl Phosphate Substrate Systems, Alkaline Phosphatase, Alphabetical Listing, Bioassay, Colorimetric,
Detection, Detection Substrates, ELISA Substrates, Enzyme Substrates, Plant Biotechnology, Substrates, Substrates by Enzyme, Substrates for Alkaline Phosphatase, Substrates, Buffers & Blockers, Tablets, 分子生物学, 抗体, 植物免疫化学, 生化试剂, 辅助产品, 酶、抑制剂和底物
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form tablet
storage temp. −20°C
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文献和实验Cloning of small RNAs with 5’ phosphate and 3’ OH ends
ladder already prepared (sizes marked 20, 30, 40, 60 and 80) Run the gel at 150V until the bromophenol blue reaches the bottom of the gel (about 2 hours). Stain the ladder portion of the gel with 0.5X EtBr for 2-5 min. Cut out the gel
ELISA PROTOCOL 【Yale University 】
-nitrophenyl phosphate (5 mg/tablet). SigmaCat.# N93895) AP-conjugated secondary Ab: AP-Rabbit anti-mouse IgG(Zymed). Reconstitute in ddH2O as prescribed by manufacturer andaliquot and store at -70 °C.6) 100 mM EDTA pH
ELISA PROTOCOL（Yale University）
1. Materials (1) TS Buffer: 10 mM Tris pH 8.0 150 mM NaCl (2) TST Buffer: TS Buffer with 0.05% Tween-20 (3) Alkaline Phosphatase Buffer: 50 mM NaCarbonate 1 mM MgCl2 (Bring to pH 9.8 with 1 N HCl) (4) AP Substrate: p-nitrophenyl phosphate (5 mg
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