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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
dense products have been devised. A chromogenic substrate, 5-bromo-4-chloro-3-indolyl phosphate (X-Phos), which is very similar to X-gal, can be employed for detection and leads to production of a blue precipitate (Figure 1). In addition, the tetrazolium
Human Placental Alkaline Phosphatase Stain
off staining solution with water. STOCK SOLUTIONS XPHOS - 100X solution is 10 mg/ml 5-bromo-4-chloro-3-indolyl phosphate, disodium salt (Sigma) in water, store at -20°C. NBT - 100X solution is 50 mg/ml Nitro Blue tetrazolium (Sigma) in 70
Modified Multiple Primer Extension Method
. The products were ultimately detected by carrying out a colorimetric reaction with a substrate solution containing 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium. We have further successfully combined this method with multiplex PCR. We demonstrate
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