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- YB553Mu02
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- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
Recombinant Matrix Metalloproteinase 9 (MMP9)
- 库存：
10000
- 供应商：
钰博生物
基质金属蛋白酶9(MMP9)重组蛋白
适用生物 Mus musculus (Mouse，小鼠)
基质金属蛋白酶9(MMP9)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 n/a 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
ybA156Hu01 糖抗原19-9(CA19-9)重组蛋白 Recombinant Carbohydrate Antigen 19-9 (CA19-9) Homo sapiens (Human)
ybA377Hu01 视黄醇结合蛋白2(RBP2)重组蛋白 Recombinant Retinol Binding Protein 2, Cellular (RBP2) Homo sapiens (Human)
ybA388Mu01 补体成分5a(C5a)重组蛋白 Recombinant Complement Component 5a (C5a) Mus musculus (Mouse)
ybA395Bo01 抑制素A(INHA)重组蛋白 Recombinant Inhibin A (INHA) Bos taurus; Bovine (Cattle)
ybA421Ra01 髓鞘少突胶质细胞糖蛋白(MOG)重组蛋白 Recombinant Myelin Oligodendrocyte Glycoprotein (MOG) Rattus norvegicus (Rat)
ybA430Ra01 沉默调节蛋白2(SIRT2)重组蛋白 Recombinant Sirtuin 2 (SIRT2) Rattus norvegicus (Rat)
ybA437Mu01 雌激素受体β(ERβ)重组蛋白 Recombinant Estrogen Receptor Beta (ERb) Mus musculus (Mouse)
ybA449Hu02 胱天蛋白酶7(CASP7)重组蛋白 Recombinant Caspase 7 (CASP7) Homo sapiens (Human)
ybA472Ga01 降钙素(CT)重组蛋白 Recombinant Calcitonin (CT) Chicken (Gallus)
ybA476Ra01 半胱氨酸蛋白酶抑制剂A(CSTA)重组蛋白 Recombinant Cystatin A (CSTA) Rattus norvegicus (Rat)
ybA523Hu01 血小板衍生生长因子AA(PDGFAA)重组蛋白 Recombinant Platelet Derived Growth Factor AA (PDGFAA) Homo sapiens (Human)
ybA547Mu02 血管细胞粘附分子1(VCAM1)重组蛋白 Recombinant Vascular Cell Adhesion Molecule 1 (VCAM1) Mus musculus (Mouse)
ybA548Hu02 细胞间粘附分子1(ICAM1)重组蛋白 Recombinant Intercellular Adhesion Molecule 1 (ICAM1) Homo sapiens (Human)
ybA568Po01 S100钙结合蛋白A11(S100A11)重组蛋白 Recombinant S100 Calcium Binding Protein A11 (S100A11) Sus scrofa; Porcine (Pig)
ybA601Mu02 髓过氧化物酶(MPO)重组蛋白 Recombinant Myeloperoxidase (MPO) Mus musculus (Mouse)
适用生物 Mus musculus (Mouse，小鼠)
基质金属蛋白酶9(MMP9)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 n/a 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Matrix Metalloproteinase 9 (MMP9)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Mus musculus (Mouse) |
| Product No. | RPA553Mu02 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | n/a |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
基质金属蛋白酶9(MMP9)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
基质金属蛋白酶9(MMP9)重组蛋白Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
ybA133Ov01 肿瘤坏死因子α(TNFα)重组蛋白 Recombinant Tumor Necrosis Factor Alpha (TNFa) Ovis aries; Ovine (Sheep)
基质金属蛋白酶9(MMP9)重组蛋白Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
ybA156Hu01 糖抗原19-9(CA19-9)重组蛋白 Recombinant Carbohydrate Antigen 19-9 (CA19-9) Homo sapiens (Human)
ybA377Hu01 视黄醇结合蛋白2(RBP2)重组蛋白 Recombinant Retinol Binding Protein 2, Cellular (RBP2) Homo sapiens (Human)
ybA388Mu01 补体成分5a(C5a)重组蛋白 Recombinant Complement Component 5a (C5a) Mus musculus (Mouse)
ybA395Bo01 抑制素A(INHA)重组蛋白 Recombinant Inhibin A (INHA) Bos taurus; Bovine (Cattle)
ybA421Ra01 髓鞘少突胶质细胞糖蛋白(MOG)重组蛋白 Recombinant Myelin Oligodendrocyte Glycoprotein (MOG) Rattus norvegicus (Rat)
ybA430Ra01 沉默调节蛋白2(SIRT2)重组蛋白 Recombinant Sirtuin 2 (SIRT2) Rattus norvegicus (Rat)
ybA437Mu01 雌激素受体β(ERβ)重组蛋白 Recombinant Estrogen Receptor Beta (ERb) Mus musculus (Mouse)
ybA449Hu02 胱天蛋白酶7(CASP7)重组蛋白 Recombinant Caspase 7 (CASP7) Homo sapiens (Human)
ybA472Ga01 降钙素(CT)重组蛋白 Recombinant Calcitonin (CT) Chicken (Gallus)
ybA476Ra01 半胱氨酸蛋白酶抑制剂A(CSTA)重组蛋白 Recombinant Cystatin A (CSTA) Rattus norvegicus (Rat)
ybA523Hu01 血小板衍生生长因子AA(PDGFAA)重组蛋白 Recombinant Platelet Derived Growth Factor AA (PDGFAA) Homo sapiens (Human)
ybA547Mu02 血管细胞粘附分子1(VCAM1)重组蛋白 Recombinant Vascular Cell Adhesion Molecule 1 (VCAM1) Mus musculus (Mouse)
ybA548Hu02 细胞间粘附分子1(ICAM1)重组蛋白 Recombinant Intercellular Adhesion Molecule 1 (ICAM1) Homo sapiens (Human)
ybA568Po01 S100钙结合蛋白A11(S100A11)重组蛋白 Recombinant S100 Calcium Binding Protein A11 (S100A11) Sus scrofa; Porcine (Pig)
ybA601Mu02 髓过氧化物酶(MPO)重组蛋白 Recombinant Myeloperoxidase (MPO) Mus musculus (Mouse)
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文献和实验相关实验
【求助】关于培养中细胞的基质金属蛋白酶(MMPs)的检测问题
topicare 逼人行将做一个关于基质金属蛋白酶（MMPs）的实验，阅读了一些文献，发现MMPs的功能较多，其中一个重要的作用就是在恶性肿瘤的侵袭和转移中发挥重要作用。此外MMPs的多水平调节也相当复杂，但是，目前已有的研究表明激素、生长因子、癌基因和细胞周期素均可影响MMPs的转录，其中IL-1、IL-12、表皮生长因子(EGF)、血小板衍生生长因子(PDGF)、转化生长因子α(TGFα)、肿瘤坏死因子α(TNFα)均能上调MMPs的表达，而转化生长因子β
隆抗体蛋白产率的下限，即1-30μg/l08细胞/24小时。有人认为其限速步骤可嚣是在工程细胞中(对于重组蛋白来讲，常是异源的)，重组蛋白的分泌效率较低。1、表达载体（1）表达栽体的类型哺乳动物细胞表达外源重组蛋白可利用质粒转染和病毒载体的感染。利用质粒转染获得稳定的转染细胞需几周甚至几个月时间，而利用病毒表达系统则可快速感染细胞，在几天内使外源基因整合到病毒载体中，尤其适用于从大量表达产物中检测出目的蛋白。根据进入宿主细胞的方式，可将表达载体分为病毒载体与质粒载体。病毒载体是以病毒颗粒的方式，通过病毒
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基质金属蛋白酶9(MMP9)重组蛋白
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